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This communication reports a method for increasing the speed of separation of bound and free antigen in radioimmunoassay systems with no loss in the specificity of binding. The technique uses a mixture of second antibody and polyethylene glycol. It is not species or antibody specific, and systems using specific first antibodies from rabbit, goat or sheep are all functional. Results for the assay of parathyrin, calcitonin and corticotropin are described here, although the system has been shown to work for triiodothyronine, thyroxin, thyrotropin, thyroxine binding globulin and transferrin. The time taken for the reaction between first and second antibody is in the order of seconds, and the stability of the complex is unchanged over a period of hours.
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
June 1971,
Saishin igaku. Modern medicine,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
August 1981,
Nihon Naibunpi Gakkai zasshi,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
May 1979,
Clinical chemistry,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
November 1972,
Clinica chimica acta; international journal of clinical chemistry,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
July 1979,
Clinical chemistry,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
September 1979,
Clinical chemistry,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
November 1983,
Clinical chemistry,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
January 1974,
Archiv fur die gesamte Virusforschung,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
March 1976,
Steroids,
W G Wood, and
G Stalla, and
O A Müller, and
P C Scriba
September 1984,
Annals of clinical biochemistry,
