OBJECTIVE We tested various doses of MgSO(4) to investigate the effect of Mg on a spinal cord ischemia. METHODS Rats were treated with either MgSO(4) (30, 100, or 300 mg/kg; group Mg(low), group Mg(medium), group Mg(high), respectively, n = 10 for each) or saline (control group; n = 10) before ischemia. Spinal cord ischemia was induced using a balloon-tipped catheter placed on proximal descending aorta. During surgery, hemodynamic variables were recorded before ischemia, during aortic occlusion and after reperfusion. Neurologic function was assessed using the motor deficit index (MDI; 0 = normal, 6 = complete paralysis) until seven days after reperfusion, and histologic examination of spinal cord was performed. RESULTS After reperfusion, the mean arterial pressure in the group Mg(high) was significantly lower than other groups. Compared to the control group, the groups Mg(low) and Mg(medium) did not show any difference in MDI and the group Mg(high) showed significantly higher MDI. The number of normal motor neurons was similar among other groups except the group Mg(high) had a significantly fewer normal motor neurons. CONCLUSIONS Intravenous MgSO(4) with low or medium dose (30, or 100 mg/kg) did not improve neurological injury following spinal cord ischemia. Furthermore, higher dose of MgSO(4) (300 mg/kg) resulted in hemodynamic instability and aggravated neurologic outcome.