Species identification of autoclaved meat samples using antisera to thermostable muscle antigens in an enzyme immunoassay. 1987

E K Kang'ethe, and J M Gathuma
Department of Public Health, Pharmacology and Toxicology, College of Agriculture and Veterinary Sciences, University of Nairobi, PO Box 29053, Kabete (Nairobi), Kenya.

An enzyme immunoassay (EIA) suitable for use in identification of cooked and autoclaved meat samples using antisera to thermostable muscle antigens (TMA) is described. Goat antisera to TMA of various species were tested against homologous and heterologous partially purified thermostable muscle antigens (PTMA) in an indirect EIA. Goat anti-eland and anti-cattle TMA sera were the poorest in differentiating other species PTMAs. Identification of various species PTMAs could be achieved using a battery of goat anti-TMA sera, where homologous goat anti-TMA serum fails to differentiate some of the PTMAs tested.

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