The Affective Neuroscience Personality Scales have been designed to provide a personality assessment tool based on six distinct affective systems. The six neural systems involved were labeled PLAY, SEEK, CARE, FEAR, ANGER and SADNESS. Spirituality has been integrated into the questionnaire as a seventh dimension because, in opinion of Panksepp and his colleagues is one of the most interesting human emotion. The aim of the present paper was introduce the validation of the Spanish version of Affective Neuroscience Personality Scales and their first psychometric results in a sample of 411 college students. Participants completed the Spanish version of ANPS, just as a personality scale of five factors (NEO-FFI-R), and the Scales of Positive and Negative Affect (PANAS). The factor structure obtained and psychometric properties of the scales indicate that the Spanish version of the scales provides an effective tool to measure the seven dimensions of personality proposal in the original questionnaire.