BIOMAT Database Logo BIOMAT Database Logo

Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. 2005

P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India.

Adulteration of high quality meat and meat products with their inferior/cheaper counterparts is a problem in the meat industry. The present study investigated the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene for identification of the origin of meats. PCR-RFLP was applied for species identification of beef, buffalo meat, mutton and chevon. PCR amplification yielded a 456-bp fragment in each of these species. The amplicons were digested with AluI, HhaI, ApoI and BspTI restriction enzymes resulting in a pattern that could identify and differentiate each of the above species. This technique did not yield satisfactory results with meat mixtures/meats. However, consistent results were obtained with both fresh and processed meat samples.

UI MeSH Term Description Entries

Related Publications

P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
January 2005, Critical reviews in microbiology,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Rapid Identification Method of Omphalotus japonicus by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
January 2017, Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates.
February 1999, Parasitology,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Identification of roots of woody species using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis
March 1999, Molecular ecology,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Sequence characterization and polymerase chain reaction-restriction fragment length polymorphism of the mitochondrial DNA 12S rRNA gene provides a method for species identification of Indian deer.
August 2008, Mitochondrial DNA,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Identification of screwworm species by polymerase chain reaction-restriction fragment length polymorphism.
January 1996, Medical and veterinary entomology,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Comprehensive typing of HLA-DPB1 alleles by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
December 1995, Clinical and investigative medicine. Medecine clinique et experimentale,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis.
January 2016, The Indian journal of medical research,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Identification of species in animal feedstuffs by polymerase chain reaction--restriction fragment length polymorphism analysis of mitochondrial DNA.
August 2001, Journal of agricultural and food chemistry,
P S Girish, and A S R Anjaneyulu, and K N Viswas, and B M Shivakumar, and M Anand, and M Patel, and B Sharma
Molecular identification of two distinct hemagglutinin types of measles virus by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
February 1995, Molecular and cellular probes,
Copied contents to your clipboard!