Biomaterial Database

Serological identification of viral and virus-related antigens on DBA/2 mouse leukemia lymphocytes. 1979

I Steuden, and C Radzikowski, and T Zak-Nejmark

Allogenic, semisyngeneic and syngeneic sera of animals immunized with ML-positive leukemia L1210 cells, besides anti-ML antibodies, contain antibodies which react with Gross cellular surface antigen. ML antigen and Gross cellular surface antigen were shown by the immunoferritin test in electron microscopy, and by the blocking test, to be situated on different parts of the cell surface. No budding viral particles were found on the areas occupied by these antigens. By distinguishing the ML antigen identified on the surface of leukemia L1210 cells from the Gross cellular antigen, it was shown that the MTV present in leukemias of DBA/2 mice has no leukemogenic properties. Demonstration of core and envelope antigens of the MTV and Gross MuLV, besides C particles and intracytoplasmatic A particles, which are precursors of B particles, is proof of existence of genomes of both viruses in leukemia L1210 cells. The ability of leukemia L1210 cells to absorb activity from the anti-ML sera and reaction between anti-ML sera and isolated B particles of the MTV in immunoprecipitation, indicate probable existence of an antigenic component of the MTV within the ML antigen.

UI	MeSH Term	Description	Entries
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D007939	Leukemia L1210	An experimental LYMPHOCYTIC LEUKEMIA of mice.	Leukemia L 1210,L 1210, Leukemia,L1210, Leukemia
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008324	Mammary Tumor Virus, Mouse	The type species of BETARETROVIRUS commonly latent in mice. It causes mammary adenocarcinoma in a genetically susceptible strain of mice when the appropriate hormonal influences operate.	Bittner Virus,Mammary Cancer Virus,Mouse mammary tumor virus,Mammary Tumor Viruses, Mouse
D008325	Mammary Neoplasms, Experimental	Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.	Experimental Mammary Neoplasms,Neoplasms, Experimental Mammary,Experimental Mammary Neoplasm,Mammary Neoplasm, Experimental,Neoplasm, Experimental Mammary
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D000406	AKR murine leukemia virus	A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) isolated from spontaneous leukemia in AKR strain mice.	AKR Virus,Gross Virus,AKR Viruses,Virus, AKR,Virus, Gross,Viruses, AKR
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000954	Antigens, Surface	Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.	Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral