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Recently, we have characterized a lymphocyte blastogenesis inhibitory factor (LBIF) which was purified from the culture supernatant of a human histiocytic lymphoma U-937 (Sugimura, K. et al., Eur. J. Immunol. 1989. 19: 1357). In this study, we investigated the effect of LBIF on the antibody production of autoimmune MRL mice in vitro. We demonstrated here that (a) LBIF inhibited the IgM, IgG and IgA antibody responses of lipopolysaccharide (LPS)-stimulated spleen cells of normal BALB/c mice, (b) in the case of old autoimmune MRL/Mp-lpr/lpr (MRL/l) and MRL/Mp-(+)/+ mice, however, LBIF inhibited IgM and IgA but not IgG responses of LPS-stimulated spleen cells, (c) the antibody production of all IgG subclasses, IgG3, IgG1, IgG2b and IgG2a, was not sensitive to LBIF inhibitory activity in these autoimmune mice, (d) in young MRL mice (3-5-week-old MRL/l), which were phenotypically normal, LPS-induced antibody production of all isotypes (IgM, IgG and IgA) was strongly inhibited by LBIF as shown in normal BALB/c mice and (e) in the case of 7-week-old MRL/l the insensitivity to LBIF was concomitant with the appearance of gamma + B lymphocytes. Thus, by employing LBIF as a probe, this study showed a correlation between the pathogenesis of MRL autoimmune disease and the lack of LBIF sensitivity of hyperactive B lymphocytes and suggested that the intrinsic abnormality of autoimmune MRL B lymphocytes might be confined to gamma- but not mu- or alpha-committed B cells.
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
May 1976,
Blut,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
January 2003,
In vivo (Athens, Greece),
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
May 1989,
Clinical and experimental immunology,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
July 2006,
International immunology,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
November 1993,
Physiology & behavior,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
November 2005,
Journal of leukocyte biology,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
February 2003,
Brain research,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
October 1983,
Clinical immunology and immunopathology,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
July 1990,
The Journal of experimental medicine,
K Sugimura, and
Y Wada, and
T Kimura, and
T Ohno, and
S Kobayashi, and
I Azuma
July 1984,
European journal of immunology,
