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The properties of cells isolated from a Leydig cell tumour have been compared with normal rat testis Leydig cells. These cells were found to be similar in the following respects: 1. Lutropin-stimulated cyclic AMP and testosterone production. 2. Lutropin-activated protein kinase activity followed by phosphorylation of endogenous proteins of mol. wts. 57,000and 14,000. 3. Parallel lutropin dose vs. response curves for phosphorylation of the endogenous proteins and for testosterone production. 4. Two forms of isoenzyme, cyclic AMP dependent protein kinase, present. They differed mainly with respect to the lutropin-stimulated testosterone production, which was much lower in the tumour cells compared with the normal adult testis Leydig cells (4.6 +/- 1.1 and 114 +/- 16 ng testosterone/10(6) cells per 2 h, respectively). However, the lutropin-stimulated steroid production in the tumour cells was quantitatively comparable with the normal rat Leydig cell when the metabolism of pregnenolone in intact cells and mitochondria was inhibited by addition of SU-10603 and/or cyanoketone. It is concluded that the Leydig cell tumour used in this study can be used to investigate certain aspects of lutropin action where large quantities of cells are required.
B A Cooke, and
L M Lindh, and
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M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
February 1981,
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B A Cooke, and
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F H Janszen, and
M J van Driel, and
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M P van der Plank, and
H J van der Molen
February 1980,
Acta endocrinologica,
B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
May 1982,
The Biochemical journal,
B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
March 1982,
The Biochemical journal,
B A Cooke, and
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M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
May 1985,
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B A Cooke, and
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C P Bakker, and
M P van der Plank, and
H J van der Molen
September 1990,
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B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
March 1985,
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B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
August 1981,
The Biochemical journal,
B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
January 1984,
Scandinavian journal of urology and nephrology,
B A Cooke, and
L M Lindh, and
F H Janszen, and
M J van Driel, and
C P Bakker, and
M P van der Plank, and
H J van der Molen
June 1981,
The Biochemical journal,
