Flame atomic absorption spectrometric determination of serum zinc: collaborative study. 1990

D F Perry
University of Arizona, Department of Veterinary Sciences, Tucson 85721.

A method for determining serum zinc concentrations was collaboratively studied by 9 laboratories. Serum samples in polypropylene tubes are directly diluted with 0.03% Brij 35, and zinc is determined by flame atomic absorption spectrophotometry (AAS). A certified standard reference material serves as an external control. Repeatability relative standard deviations (RSDr) were 1.2 and 11.4% and reproducibility relative standard deviations (RSDR) were 6.1 and 12.9% for serum zinc concentrations of 6.36 and 0.63 micrograms/mL, respectively. The method has been approved interim official first action by AOAC.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013054 Spectrophotometry, Atomic Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed. Spectrophotometry, Atomic Absorption,AA Spectrophotometry,AE Spectrophotometry,Atomic Absorption Spectrophotometry,Atomic Emission Spectrophotometry,Atomic Spectrophotometry,Inductively Coupled Plasma Atomic Emission Spectrophotometry,Inductively Coupled Plasma Atomic Emission Spectroscopy,Spectrophotometry, Atomic Emission,AA Spectrophotometries,AE Spectrophotometries,Absorption Spectrophotometry, Atomic,Emission Spectrophotometry, Atomic,Spectrophotometries, AA,Spectrophotometries, AE,Spectrophotometry, AA,Spectrophotometry, AE
D015032 Zinc A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.

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