-transparent.png){kind=logo}
The HSV thymidine kinase genetic system has a number of advantages for the study of virus and cell genetics. Both forward and reverse mutants are readily selected. The enzyme can be assayed directly in the presence of the cell TK by the choice of the appropriate substrate, for example, 125IdC. The gene product, HSV TK, can be identified by SDS-polyacrylamide gel electrophoresis and mutants can be found with alterations in the electrophoretogram. The in vitro synthesis of HSV TK in response to HSV mRNA has been achieved and can be used to analyse specific mutant virus.
Related Publications
K J Cremer, and
W P Summers, and
W C Summers
January 2008,
The open biochemistry journal,
K J Cremer, and
W P Summers, and
W C Summers
April 2008,
Virology,
K J Cremer, and
W P Summers, and
W C Summers
January 1981,
Nature,
K J Cremer, and
W P Summers, and
W C Summers
March 1981,
Proceedings of the National Academy of Sciences of the United States of America,
K J Cremer, and
W P Summers, and
W C Summers
January 1983,
Gene,
K J Cremer, and
W P Summers, and
W C Summers
December 1982,
The Journal of general virology,
K J Cremer, and
W P Summers, and
W C Summers
April 1996,
Virology,
K J Cremer, and
W P Summers, and
W C Summers
April 1981,
Proceedings of the National Academy of Sciences of the United States of America,
K J Cremer, and
W P Summers, and
W C Summers
November 1998,
Antimicrobial agents and chemotherapy,
K J Cremer, and
W P Summers, and
W C Summers
July 1987,
Antimicrobial agents and chemotherapy,
