BIOMAT Database Logo BIOMAT Database Logo

Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. 2012

Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France.

We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T lymphocytes at 20°C and 37°C by Single Particle Tracking using Quantum Dots. We found that the receptors presented two major distinct behaviors that were not equally affected by temperature changes. About half of the receptors showed a random diffusion with a diffusion coefficient increasing upon raising the temperature. The other half of the receptors was permanently or transiently confined with unchanged dynamics on raising the temperature. These observations suggest that two distinct subpopulations of CD4 receptors with different environments are present at the surface of living T lymphocytes.

UI MeSH Term Description Entries
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D006678 HIV Human immunodeficiency virus. A non-taxonomic and historical term referring to any of two species, specifically HIV-1 and/or HIV-2. Prior to 1986, this was called human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). From 1986-1990, it was an official species called HIV. Since 1991, HIV was no longer considered an official species name; the two species were designated HIV-1 and HIV-2. AIDS Virus,HTLV-III,Human Immunodeficiency Viruses,Human T-Cell Lymphotropic Virus Type III,Human T-Lymphotropic Virus Type III,LAV-HTLV-III,Lymphadenopathy-Associated Virus,Acquired Immune Deficiency Syndrome Virus,Acquired Immunodeficiency Syndrome Virus,Human Immunodeficiency Virus,Human T Cell Lymphotropic Virus Type III,Human T Lymphotropic Virus Type III,Human T-Cell Leukemia Virus Type III,Immunodeficiency Virus, Human,Immunodeficiency Viruses, Human,Virus, Human Immunodeficiency,Viruses, Human Immunodeficiency,AIDS Viruses,Human T Cell Leukemia Virus Type III,Lymphadenopathy Associated Virus,Lymphadenopathy-Associated Viruses,Virus, AIDS,Virus, Lymphadenopathy-Associated,Viruses, AIDS,Viruses, Lymphadenopathy-Associated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D015704 CD4 Antigens 55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. They are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. T4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120. Antigens, CD4,CD4 Molecule,CD4 Receptor,CD4 Receptors,Receptors, CD4,T4 Antigens, T-Cell,CD4 Antigen,Receptors, Surface CD4,Surface CD4 Receptor,Antigen, CD4,Antigens, T-Cell T4,CD4 Receptor, Surface,CD4 Receptors, Surface,Receptor, CD4,Surface CD4 Receptors,T-Cell T4 Antigens,T4 Antigens, T Cell
D019169 Jurkat Cells A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation. Cell, Jurkat,Cells, Jurkat,Jurkat Cell

Related Publications

Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Distinct gamma/delta T cell receptors define two subsets of circulating porcine CD2-CD4-CD8- T lymphocytes.
February 1990, European journal of immunology,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments.
November 2017, Nanomaterials (Basel, Switzerland),
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Two distinct Fc receptors for IgG on human peripheral T lymphocytes.
July 1982, Scandinavian journal of immunology,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Two molecularly independent surface receptors identify bovine T lymphocytes.
January 1981, Journal of immunological methods,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Single-Particle Tracking of Cell Surface Proteins.
January 2018, Methods in molecular biology (Clifton, N.J.),
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Uses of single-particle tracking in living cells.
April 2010, Drug discoveries & therapeutics,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Single particle tracking with sterol modulation reveals the cholesterol-mediated diffusion properties of integrin receptors.
October 2014, Physical biology,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Single-particle tracking methods for the study of membrane receptors dynamics.
September 2009, The European journal of neuroscience,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Activation of functionally distinct subsets of CD4+ T lymphocytes.
January 1991, Research in immunology,
Patrice Mascalchi, and Anne Sophie Lamort, and Laurence Salomé, and Fabrice Dumas
Distribution of erythrocyte receptors at the surface membrane of human T-lymphocytes.
April 1976, Biomedicine / [publiee pour l'A.A.I.C.I.G.],
Copied contents to your clipboard!