Biomaterial Database

Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE): site characterization and on-site bioremediation. 2012

Françoise Fayolle-Guichard, and Jonathan Durand, and Mathilde Cheucle, and Mònica Rosell, and Rory Julien Michelland, and Jean-Philippe Tracol, and Françoise Le Roux, and Geneviève Grundman, and Olivier Atteia, and Hans H Richnow, and Alain Dumestre, and Yves Benoit

IFP Energies nouvelles, 1 et 4 avenue de Bois-Préau, 92852 Rueil-Malmaison, France. francoise.fayolle@ifpen.fr

Ethyl tert-butyl ether (ETBE) was detected at high concentration (300mgL(-1)) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE: 0.91 and 0.83 mg L(-1)h(-1) and BTEX: 0.64 and 0.82 mg L(-1)h(-1), respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74 mg L(-1)). An on-site pilot plant (2m(3)) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5×10(6) copies of ethB gene per L(-1).

UI	MeSH Term	Description	Entries
D005019	Ethyl Ethers	Organic compounds having ethyl groups bound to an oxygen atom.	Ethoxy Compounds,Compounds, Ethoxy,Ethers, Ethyl
D005602	France	A country in western Europe bordered by the Atlantic Ocean, the English Channel, the Mediterranean Sea, and the countries of Belgium, Germany, Italy, Spain, Switzerland, the principalities of Andorra and Monaco, and by the duchy of Luxembourg. Its capital is Paris.	Corsica,Saint Pierre and Miquelon,Miquelon and Saint Pierre,Miquelon and St. Pierre,St. Pierre and Miquelon
D005742	Gasoline	Volative flammable fuel (liquid hydrocarbons) derived from crude petroleum by processes such as distillation reforming, polymerization, etc.	Diesel Fuel,Diesel Fuels,Fuel, Diesel,Fuels, Diesel,Gasolines
D001673	Biodegradation, Environmental	Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.	Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D012240	Rhodococcus	A bacterial genus of the order ACTINOMYCETALES.
D014872	Water Movements	The flow of water in enviromental bodies of water such as rivers, oceans, water supplies, aquariums, etc. It includes currents, tides, and waves.	Movement, Water,Movements, Water,Water Movement
D014874	Water Pollutants, Chemical	Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water.	Chemical Water Pollutants,Landfill Leachate,Leachate, Landfill,Pollutants, Chemical Water
D060587	Groundwater	Liquid water present beneath the surface of the earth.	Aquifers,Ground Water,Ground-Water,Subterranean Lakes,Underground Lakes,Underground Streams,Underground Water,Water Table,Aquifer,Ground Waters,Ground-Waters,Groundwaters,Lake, Subterranean,Lake, Underground,Lakes, Subterranean,Lakes, Underground,Stream, Underground,Streams, Underground,Subterranean Lake,Table, Water,Tables, Water,Underground Lake,Underground Stream,Underground Waters,Water Tables,Water, Underground,Waters, Underground
D018508	Water Purification	Any of several processes in which undesirable impurities in water are removed or neutralized; for example, chlorination, filtration, primary treatment, ion exchange, and distillation. It includes treatment of WASTEWATER to provide potable and hygienic water in a controlled or closed environment as well as provision of public drinking water supplies.	Waste Water Purification,Waste Water Treatment,Wastewater Purification,Wastewater Treatment,Water Treatment,Purification, Waste Water,Purification, Wastewater,Purification, Water,Treatment, Waste Water,Treatment, Wastewater,Treatment, Water,Waste Water Purifications,Waste Water Treatments,Water Purification, Waste
D019117	Burkholderia	A genus of gram-negative, aerobic, rod-shaped bacteria originally classified as members of the PSEUDOMONADACEAE.