-transparent.png){kind=logo}
The chain length of [3H]hyaluronic acid synthesized by cultivating human skin fibroblasts in the presence of [3H]glucosamine was investigated. [3H]Hyaluronic acid obtained from the matrix fraction was excluded from a Sepharose CL-2B column irrespective of the incubation period, whereas that from the medium was depolymerized into a constant chain length (Mr = 40,000). The reducing and non-reducing terminals of the depolymerized hyaluronic acid were N-acetylglucosamine and glucuronic acid, respectively. Prolonged incubation produced no oligosaccharides as shown by examination of hyaluronidase digests, suggesting the presence of a novel endo-beta-N-acetylglucosaminidase in cultured human skin fibroblasts.
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
November 1978,
Lipids,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
November 1981,
Journal of cellular physiology,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
May 2005,
Pharmacological research,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
December 1992,
Biochimica et biophysica acta,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
January 2015,
Drug design, development and therapy,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
April 1980,
Biochimica et biophysica acta,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
October 1972,
Israel journal of medical sciences,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
May 1990,
The Journal of investigative dermatology,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
June 1994,
International journal of biological macromolecules,
T Nakamura, and
K Takagaki, and
K Kubo, and
A Morikawa, and
S Tamura, and
M Endo
March 1990,
The Journal of clinical endocrinology and metabolism,
