Clusterin mRNA and protein in Alzheimer's disease. 2012

Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
Dementia Research Group, Institute of Clinical Neurosciences, University of Bristol, Frenchay Hospital, Bristol, UK. shabnam.baig@bristol.ac.uk

Clusterin, a multifunctional lipoprotein is expressed in a number of tissues but expression is particularly high in the brain, where it binds to amyloid-β (Aβ), possibly facilitating Aβ transport into the bloodstream. Its concentration in peripheral blood was identified as a potential biomarker for Alzheimer's disease (AD) and predicted retention of (11)C-Pittsburgh Compound B in the temporal lobe. Single-nucleotide polymorphisms in the clusterin gene, CLU, are associated with the risk of developing AD. We measured clusterin mRNA levels in control and AD brains and investigated the relationship of the clusterin protein to soluble, insoluble, and plaque-associated Aβ. Clusterin mRNA levels were unchanged when normalized to GAPDH but modestly increased in the frontal and temporal cortex in AD in relation to NSE and MAP-2. Levels of NSE and MAP-2 mRNA were reduced in the AD frontal cortex. Clusterin protein concentration was unchanged and did not correlate with the amount of Aβ present. In the frontal cortex, clusterin concentration was higher in APOE ε4-negative brains but no effect of APOE was detected in the temporal cortex or thalamus. Overall clusterin mRNA and protein levels are unaltered in the neocortex in AD and clusterin concentration does not reflect Aβ content. The increase in clusterin noted in peripheral blood in AD may reflect increased passage of this chaperone protein across the blood-brain barrier but further work is needed to determine how CLU variants influence the development of AD.

UI MeSH Term Description Entries
D008297 Male Males
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly
D000369 Aged, 80 and over Persons 80 years of age and older. Oldest Old
D000544 Alzheimer Disease A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57) Acute Confusional Senile Dementia,Alzheimer's Diseases,Dementia, Alzheimer Type,Dementia, Senile,Presenile Alzheimer Dementia,Senile Dementia, Alzheimer Type,Alzheimer Dementia,Alzheimer Disease, Early Onset,Alzheimer Disease, Late Onset,Alzheimer Sclerosis,Alzheimer Syndrome,Alzheimer Type Senile Dementia,Alzheimer's Disease,Alzheimer's Disease, Focal Onset,Alzheimer-Type Dementia (ATD),Dementia, Presenile,Dementia, Primary Senile Degenerative,Early Onset Alzheimer Disease,Familial Alzheimer Disease (FAD),Focal Onset Alzheimer's Disease,Late Onset Alzheimer Disease,Primary Senile Degenerative Dementia,Senile Dementia, Acute Confusional,Alzheimer Dementias,Alzheimer Disease, Familial (FAD),Alzheimer Diseases,Alzheimer Type Dementia,Alzheimer Type Dementia (ATD),Alzheimers Diseases,Dementia, Alzheimer,Dementia, Alzheimer-Type (ATD),Familial Alzheimer Diseases (FAD),Presenile Dementia,Sclerosis, Alzheimer,Senile Dementia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D051152 Clusterin A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological disturbance states such as CANCER; APOPTOSIS; and various NEUROLOGICAL DISORDERS. Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE. ApoJ Protein,Apolipoprotein J,Complement Lysis Inhibitor,Complement-Associated Protein SP-40,40,Ionizing Radiation-Induced Protein-8,MAC393 Antigen,SGP-2 Protein,SP 40,40 Protein,Sulfated Glycoprotein 2,Sulfated Glycoprotein-2,TRPM-2 Protein,Testosterone-Repressed Prostate Message-2 Protein,X-Ray-Inducible Protein 8,XIP8 Protein,Complement Associated Protein SP 40,40,Ionizing Radiation Induced Protein 8,Radiation-Induced Protein-8, Ionizing,SGP 2 Protein,SP-40,40, Complement-Associated Protein,TRPM 2 Protein,Testosterone Repressed Prostate Message 2 Protein,X Ray Inducible Protein 8
D019751 Intramolecular Transferases Enzymes of the isomerase class that catalyze the transfer of acyl-, phospho-, amino- or other groups from one position within a molecule to another. EC 5.4. Mutases,Transferases, Intramolecular

Related Publications

Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2005, Sub-cellular biochemistry,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2012, Advances in clinical chemistry,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
September 2012, European journal of epidemiology,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
August 2000, Microscopy research and technique,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2021, Frontiers in molecular biosciences,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
October 2009, Brain research reviews,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2023, Frontiers in aging neuroscience,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2011, Journal of Alzheimer's disease : JAD,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
December 1998, Experimental neurology,
Shabnam Baig, and Laura E Palmer, and Michael J Owen, and Julie Williams, and Patrick G Kehoe, and Seth Love
January 2012, PloS one,
Copied contents to your clipboard!