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The amino acid composition of metalloproteinase was determined. It was shown that the enzyme is made up of four cysteinyl residues which makes it distinct from other known neutral metalloproteinases from Bacillus brevis, Bacillus subtilis and thermolysine that are devoid of cysteinyl residues. The inhibiting effect of amino acids and some di- and tripeptides on the metalloproteinase activity was studied. The pH-dependence of the Michaelis constant (pKm0) of native and diethylpyrocarbonate-modified metalloproteinase (pKm) suggests that the enzyme active center contains two imidazole groups of histidine with pK alpha 1 = 6.75 +/- 0.1 and pK alpha 2 = 5.4 +/- 0.1. The experimental results are compared to those obtained with other microbial metalloproteinases.
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
April 1996,
Biokhimiia (Moscow, Russia),
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
June 1986,
Biokhimiia (Moscow, Russia),
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
January 1990,
Prikladnaia biokhimiia i mikrobiologiia,
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
September 1988,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
February 1989,
Biokhimiia (Moscow, Russia),
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
June 1969,
Antibiotiki,
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
January 1926,
The Biochemical journal,
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
February 1991,
Biophysical chemistry,
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
February 2016,
The protein journal,
V I Krupianko, and
A I Severin, and
A I Kudriavtseva, and
A Ia Valiakhmetov, and
A G Kozlovskiĭ, and
I S Kulaev
June 1974,
Life sciences,
