-transparent.png){kind=logo}
Syrian hamster embryo fibroblasts were oncogenically transformed by UV-inactivated Herpes simplex type 2. Eighteen clones were isolated shortly after transformation occurred. Two clones and their tumor derivatives were studied using several techniques. The karyotype analysis revealed different chromosome patterns in the two clones and a tendency toward hypodiploidy in the tumor derivatives. All of these cell lines were shown by molecular hybridization to contain 40% of the HSV-genome in several copies. The viral DNA sequence complexity was retained in the tumor derivatives, but a decrease in the copy number was observed. Viral RNA's were detected by in situ hybridization in all the lines that were tested. Viral antigens could be observed in these transformed cells by immunofluorescence. Finally, polypeptide analysis showed three differences between normal and transformed cells.
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
July 1980,
Virology,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
January 1977,
Proceedings of the National Academy of Sciences of the United States of America,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
January 1983,
Voprosy virusologii,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
February 1980,
Proceedings of the National Academy of Sciences of the United States of America,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
January 1973,
Intervirology,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
October 1976,
Acta virologica,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
June 1972,
Journal of virology,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
January 1980,
Intervirology,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
March 1976,
Infection and immunity,
A Kessous, and
V Bibor-Hardy, and
M Suh, and
R Simard
April 1984,
Gan,
