Biomaterial Database

Defining the contribution of select neuropeptides and their receptors in regulating sesquiterpenoid biosynthesis by Drosophila melanogaster ring gland/corpus allatum through RNAi analysis. 2012

Christine Wang, and Jinrui Zhang, and Stephen S Tobe, and William G Bendena

Department of Biology, Queen's University, Kingston, ON, Canada K7L 3N6.

The larval ring gland and adult corpus allatum (CA) of Drosophila melanogaster produce at least three sesquiterpenoid products: methyl farnesoate (MF), juvenile hormone III (JHIII), and JHIII bisepoxide (JHB(3)). Our understanding of neuropeptide regulation of sesquiterpenoid biosynthesis in D. melanogaster has been hampered by uncertainty over the biosynthetic pathway and the sites of action of regulators. As an approach to defining the neuropeptide regulators, we have used in vivo gene-specific silencing (RNAi). D. melanogaster strains containing an inducible UAS-RNAi construct made to either PheGlyLeu-NH(2)-allatostatin (FGLa/AST) and its cognate receptors Dar-1 and Dar-2 or PISCF-allatostatin (PISCF/AST) or its cognate receptors Drostar-1 or Drostar-2 were expressed in vivo. MF, JHIII and JHB(3) production was measured in ring glands of 3rd instars or corpora allata (CA) of adult females using the radiochemical assay. Reduction in FGLa/AST and Dar-1 or Dar-2 mRNA levels had no effect on MF, JHIII, or JHB(3) production in larvae or adults. Inhibition of Drostar-1 expression resulted in a significant decrease in MF and JHB(3) production in 3rd instars with little effect on JHIII biosynthesis. In contrast, inhibition of Drostar-1 in adult females led to a significant increase in MF and JHIII production. Inhibition of Drostar-2 also reduced MF biosynthesis in 3rd instars. In adults, inhibition of Drostar-2 led to a significant increase in MF and JHIII production but showed no effect on JHB(3). PISCF/AST had no effect on sesquiterpenoid biosynthesis when incubated with 3rd instar ring glands but was stimulatory when incubated with adult glands. Inhibition of short neuropeptide F (sNPF) expression by RNAi or application of sNPF to ring glands had no effect on MF, JHIII, or JHB3 biosynthesis in larvae or adults. Reduction in the neuropeptide Y receptor (NepYr) or neuropeptide F receptor (NPF-R) inhibited JHIII and JHB(3) production in 3rd instars but only reduction in NepYr resulted in JHB(3) reduction in adults.

UI	MeSH Term	Description	Entries
D007605	Juvenile Hormones	Compounds, either natural or synthetic, which block development of the growing insect.	Insect Growth Regulator,Insect Growth Regulators,Juvenile Hormone,Growth Regulators, Insect,Regulators, Insect Growth,Growth Regulator, Insect,Hormone, Juvenile,Hormones, Juvenile,Regulator, Insect Growth
D009479	Neuropeptides	Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells.	Neuropeptide
D003335	Corpora Allata	Paired or fused ganglion-like bodies in the head of insects. The bodies secrete hormones important in the regulation of metamorphosis and the development of some adult tissues.	Corpus Allatum,Corpus Cardiacum,Allata, Corpora,Allatum, Corpus
D004331	Drosophila melanogaster	A species of fruit fly frequently used in genetics because of the large size of its chromosomes.	D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D005260	Female		Females
D005786	Gene Expression Regulation	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.	Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012313	RNA	A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)	RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D012717	Sesquiterpenes	Fifteen-carbon compounds formed from three isoprenoid units with general formula C15H24.	Farnesanes,Farnesene,Farnesenes,Sesquiterpene,Sesquiterpene Derivatives,Sesquiterpenoid,Sesquiterpenoids,Derivatives, Sesquiterpene
D043562	Receptors, G-Protein-Coupled	The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.	G Protein Coupled Receptor,G-Protein-Coupled Receptor,G-Protein-Coupled Receptors,G Protein Coupled Receptors,Receptor, G-Protein-Coupled,Receptors, G Protein Coupled