[HLA antigens on erythrocytes. III. Detection using the absorption and bromelin test]. 1990

A Májský
Ustav hematologie a krevní transfúze, Praha.

HLA antigens on erythrocytes were assessed concurrently by three tests--the indirect antiglobulin, two-stage bromelin and absorption test--using polyspecific and monospecific sera against HLA antigens A1, A2, B7, B8 and B12. Evidence obtained by the bromelin test was successful only in rare instances. The positivity of the absorption test increased with the number of saturations: after triple saturation of the HLA serum with erythrocytes of the same subject readings were obtained twice to three times more frequently than after single absorption. As compared with the indirect antiglobulin test, the positive erythrocyte reactions in the absorption test were less frequent: with polyspecific HLA sera in 36.97% as compared with 78.15%, with monospecific HLA sera in 63.64% as compared with 100%. The absorption test is a suitable method for detection of HLA antigens on erythrocytes, supplementing the indirect antiglobulin test.

UI MeSH Term Description Entries
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006680 HLA Antigens Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases. Human Leukocyte Antigen,Human Leukocyte Antigens,Leukocyte Antigens,HL-A Antigens,Antigen, Human Leukocyte,Antigens, HL-A,Antigens, HLA,Antigens, Human Leukocyte,Antigens, Leukocyte,HL A Antigens,Leukocyte Antigen, Human,Leukocyte Antigens, Human
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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