Changes in 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase messenger ribonucleic acid, activity and protein levels during the estrous cycle in the bovine ovary. 1990

J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada.

A 1169 base pair fragment of bovine 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) cDNA was used to quantitate 3 beta-HSD messenger RNA (mRNA) levels in the bovine ovary during the estrous cycle. The content of 3 beta-HSD protein was measured by immunoblot analysis using an antiserum developed in rabbits against human 3 beta-HSD, whereas 3 beta-HSD activity was measured using [3H]pregnenolone, [3H] dehydroepiandrosterone, and [3H]androst-5-ene-3 beta,17 beta-diol as substrates. There was a parallel increase in 3 beta-HSD mRNA, protein content, and enzymatic activity levels from days 1-3 after estrus to maximal values at 50-100% above control on days 8-11 after estrus. Thereafter, all values decreased progressively until days 16-17 before a dramatic fall to 5% or less than maximal values on days 18-20 after estrus. Almost superimposable results of enzymatic activity were obtained with the three substrates, thus suggesting a unique 3 beta-HSD or parallel changes in the activity of multiple 3 beta-HSDs. The above-described changes observed during the luteal phase are almost exclusively due to variations in corpora lutea. In fact, 3 beta-HSD activity in ovarian follicles was approximately 10,000 lower than that measured in corpora lutea. The close correlation observed over a wide range of 3 beta-HSD mRNA, protein content, and activity levels suggests that changes of ovarian 3 beta-HSD activity are controlled at the level of 3 beta-HSD gene expression and/or 3 beta-HSD mRNA stability.

UI MeSH Term Description Entries
D009097 Multienzyme Complexes Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES. Complexes, Multienzyme
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D011375 Progesterone Reductase An enzyme that catalyzes the reduction of a 3 beta-hydroxy-delta(5)-steroid to 3-oxo-delta(4)-steroid in the presence of NAD. It converts pregnenolone to progesterone and dehydroepiandrosterone to androstenedione. EC 1.1.1.145. 3 beta-Hydroxy-delta-5-Steroid Dehydrogenase,5-Ene-3 beta-hydroxysteroid Dehydrogenase,Steroid delta(5)-3 beta-ol Dehydrogenase,3 beta Hydroxy delta 5 Steroid Dehydrogenase,5 Ene 3 beta hydroxysteroid Dehydrogenase,Dehydrogenase, 3 beta-Hydroxy-delta-5-Steroid,Dehydrogenase, 5-Ene-3 beta-hydroxysteroid,Reductase, Progesterone
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003338 Corpus Luteum The yellow body derived from the ruptured OVARIAN FOLLICLE after OVULATION. The process of corpus luteum formation, LUTEINIZATION, is regulated by LUTEINIZING HORMONE. Corpora Lutea,Lutea, Corpora
D004971 Estrus The period in the ESTROUS CYCLE associated with maximum sexual receptivity and fertility in non-primate female mammals.
D005260 Female Females
D005498 Follicular Phase The period of the MENSTRUAL CYCLE representing follicular growth, increase in ovarian estrogen (ESTROGENS) production, and epithelial proliferation of the ENDOMETRIUM. Follicular phase begins with the onset of MENSTRUATION and ends with OVULATION. Menstrual Cycle, Follicular Phase,Menstrual Cycle, Proliferative Phase,Menstrual Proliferative Phase,Preovulatory Phase,Phase, Follicular,Phase, Menstrual Proliferative,Phase, Preovulatory,Proliferative Phase, Menstrual
D006080 Ovarian Follicle An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS. Graafian Follicle,Atretic Follicle,Ovarian Follicles,Atretic Follicles,Follicle, Atretic,Follicle, Graafian,Follicle, Ovarian,Follicles, Atretic,Follicles, Graafian,Follicles, Ovarian,Graafian Follicles
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
May 1993, Biology of reproduction,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
September 1994, Biology of reproduction,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
December 1992, Endocrinology,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
May 1992, The Journal of clinical endocrinology and metabolism,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
January 1992, Endocrinology,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
January 1982, Archives of dermatological research,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
January 1990, Life sciences,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
January 1997, Skin pharmacology : the official journal of the Skin Pharmacology Society,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
February 1983, American journal of obstetrics and gynecology,
J Couët, and C Martel, and E Dupont, and V Luu-The, and M A Sirard, and H F Zhao, and G Pelletier, and F Labrie
June 1983, Steroids,
Copied contents to your clipboard!