BIOMAT Database Logo BIOMAT Database Logo

Lipoproteins in normal and atherosclerotic aorta. 1990

S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Department of Medicine, University of California, San Diego, La Jolla 92093-0613.

Each method used for the extraction and isolation of intimal lipoproteins has advantages and disadvantages. Gentle extraction methods are needed to characterize subtle modifications in the structure and biologic properties of the lipoproteins, whereas more aggressive methods are needed if the goal is to maximize the yield of lipoproteins from atherosclerotic arteries. The present paper evaluates different methods used for the isolation of intimal lipoproteins. Normal intima contains remnant-like and low density lipoprotein (LDL)-like particles that more strongly stimulate cholesterol esterification in macrophages than do control plasma LDL. Both fractions contain apolipoprotein (apo) E but neither shows clear signs of oxidative modification. LDL-like particles from atherosclerotic lesions, on the other hand, contain malondialdehyde- and 4-hydroxynonenal-lysine adducts in apo B, are chemotactic for monocytes and show increased degradation in macrophages, a process that oxidized LDL prepared in vitro can compete with. The findings support the conclusion that at least a portion of the LDL isolated from atherosclerotic lesions is similar, if not identical, to oxidatively modified LDL.

UI MeSH Term Description Entries
D008077 Lipoproteins, LDL A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues. Low-Density Lipoprotein,Low-Density Lipoproteins,beta-Lipoprotein,beta-Lipoproteins,LDL(1),LDL(2),LDL-1,LDL-2,LDL1,LDL2,Low-Density Lipoprotein 1,Low-Density Lipoprotein 2,LDL Lipoproteins,Lipoprotein, Low-Density,Lipoproteins, Low-Density,Low Density Lipoprotein,Low Density Lipoprotein 1,Low Density Lipoprotein 2,Low Density Lipoproteins,beta Lipoprotein,beta Lipoproteins
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D001161 Arteriosclerosis Thickening and loss of elasticity of the walls of ARTERIES of all sizes. There are many forms classified by the types of lesions and arteries involved, such as ATHEROSCLEROSIS with fatty lesions in the ARTERIAL INTIMA of medium and large muscular arteries. Arterioscleroses
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
[Modified lipoproteins in atherosclerotic lesions of human aorta].
February 1998, Zhonghua bing li xue za zhi = Chinese journal of pathology,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
[Stimulation of cholesterol ester synthesis in macrophages by lipoproteins from normal and atherosclerotic human aorta intima].
May 1990, Biokhimiia (Moscow, Russia),
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
[ULTRASTRUCTURE OF THE NORMAL AND ATHEROSCLEROTIC AORTA].
July 1964, Archivio "de Vecchi" per l'anatomia patologica e la medicina clinica,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Collagen types in normal and atherosclerotic human aorta.
January 1983, Indian heart journal,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Turnover of low density lipoproteins in normal and atherosclerotic rabbits.
January 1983, Acta physiologica latino americana,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
[Adiponectin in normal and atherosclerotic intima of human aorta].
January 2022, Arkhiv patologii,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Sialyltransferase activity in normal and atherosclerotic human aorta intima.
April 2001, Biochemistry. Biokhimiia,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Lipoproteins in atherosclerotic lesions. Localization by immunofluorescence of apo-low density lipoproteins in human atherosclerotic arteries from normal and hyperlipoproteinemics.
May 1975, Archives of pathology,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Cholestanol and 26-hydroxycholesterol in normal and atherosclerotic human aorta.
January 1971, Life sciences. Pt. 2: Biochemistry, general and molecular biology,
S Ylä-Herttuala, and W Palinski, and M E Rosenfeld, and D Steinberg, and J L Witztum
Localization of apolipoprotein E in normal and atherosclerotic human aorta.
December 1990, Atherosclerosis,
Copied contents to your clipboard!