Biomaterial Database

Temperature sensitivity of the arenavirus junin isolated from persistently infected Vero cells. 1979

E B Damonte, and C E Coto

A persistently infected cell culture was established from Vero cells surviving primary infection with wild-type Junin virus (JVwt). The cells were refractory to superinfection by both Junin virus and the related Tacaribe virus. Replication of virus released from the persistently infected cells (JVpi) was inhibited at 39 degrees. JVpi did not interfere with JVwt at 37 degrees, and it was inactivated at 37, 40, 43, 46 and 50 degrees much more rapidly than was JVwt.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010948	Viral Plaque Assay	Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.	Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003588	Cytopathogenic Effect, Viral	Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.	Cytopathic Effect, Viral,Viral Cytopathogenic Effect,Cytopathic Effects, Viral,Cytopathogenic Effects, Viral,Effect, Viral Cytopathic,Effect, Viral Cytopathogenic,Effects, Viral Cytopathic,Effects, Viral Cytopathogenic,Viral Cytopathic Effect,Viral Cytopathic Effects,Viral Cytopathogenic Effects
D006477	Arenaviruses, New World	One of two groups of viruses in the ARENAVIRUS genus and considered part of the New World complex. It includes JUNIN VIRUS; PICHINDE VIRUS; Amapari virus, and Machupo virus among others. They are the cause of human hemorrhagic fevers mostly in Central and South America.	Amapari virus,Chapare mammarenavirus,Hemorrhagic Fever Virus, Bolivian,Hemorrhagic Fever Viruses, American,Machupo virus,New World Arenaviruses,Sabia virus,Tacaribe virus,Allpahuayo virus,American Hemorrhagic Fever Virus,Chapare virus,Guanarito virus,Pirital virus,Tacaribe Complex Viruses,Allpahuayo viruses,Chapare mammarenaviruses,Chapare viruses,Guanarito viruses,Pirital viruses,Tacaribe viruses,viruses, Tacaribe
D001116	Arenaviridae	A family of RNA viruses naturally infecting rodents and consisting of one genus (ARENAVIRUS) with two groups: Old World Arenaviruses (ARENAVIRUSES, OLD WORLD) and New World Arenaviruses (ARENAVIRUSES, NEW WORLD). Infection in rodents is persistent and silent. Vertical transmission is through milk-, saliva-, or urine-borne routes. Horizontal transmission to humans, monkeys, and other animals is important.	Mammarenavirus,Reptarenavirus,Mammarenaviruses,Reptarenaviruses
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures