Genetic analysis of beak and feather disease virus isolated from captive psittacine birds in Thailand. 2011

Ladawan Sariya, and Phirom Prompiram, and Wacharaporn Khocharin, and Siriporn Tangsudjai, and Rassameepen Phonarknguen, and Parntep Rattanakorn, and Kridsada Chaichoun
The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand. ladawansariya@yahoo.com

Beak and feather disease virus (BFDV) is a causative agent of psittacine beak and feather disease. Genome sequences of BFDVs isolated from Thailand have not hitherto been reported. The whole genomes of 17 BFDV isolates, obtained from 12 psittacine genera, were amplified and subjected to direct sequencing revealing a length ranging from 1,990 to 2,015 nucleotides. The predicted open reading frames (ORFs) in the viral genome varied from four to six. Only ORF1, ORF2, and ORF5 were found in all isolates. Deduced amino acid sequences of BFDV ORF2 were used to construct a phylogenetic tree. The phylogram grouped BFDV into ten clusters, which showed either host species relationship or regional restriction. The Thai isolates, were grouped into three clusters, cluster I, II, and V. Cluster I and II showed restricted geographical region to Thailand, and cluster II also showed a close relationship with BFDV isolated from Australia. Cluster V demonstrated neither restricted region nor species specificity of birds. In this cluster, there was an insertion of 16 nucleotides at non coding region of all BFDV isolates. The genetic information obtained from this study can be used to help understand BFDV diversity and evolution in Thailand.

UI MeSH Term Description Entries
D011563 Psittaciformes An order of BIRDS comprised of several families and more than 300 species. It includes COCKATOOS; PARROTS; PARAKEETS; macaws; and BUDGERIGARS. Psittacines
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005241 Feathers Flat keratinous structures found on the skin surface of birds. Feathers are made partly of a hollow shaft fringed with barbs. They constitute the plumage. Feather
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013785 Thailand Formerly known as Siam, this is a Southeast Asian nation at the center of the Indochina peninsula. Bangkok is the capital city. Kingdom of Thailand,Siam
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016366 Open Reading Frames A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR). ORFs,Protein Coding Region,Small Open Reading Frame,Small Open Reading Frames,sORF,Unassigned Reading Frame,Unassigned Reading Frames,Unidentified Reading Frame,Coding Region, Protein,Frame, Unidentified Reading,ORF,Open Reading Frame,Protein Coding Regions,Reading Frame, Open,Reading Frame, Unassigned,Reading Frame, Unidentified,Region, Protein Coding,Unidentified Reading Frames
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

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