Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments. 2012

David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
Chemistry Department, University of Nebraska, Lincoln, NE 68588-0304, USA. dhage@unlserve.unl.edu

Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D007477 Ions An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D008670 Metals Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed) Metal
D011108 Polymers Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS). Polymer
D001895 Boron A trace element with the atomic symbol B, atomic number 5, and atomic weight [10.806; 10.821]. Boron-10, an isotope of boron, is used as a neutron absorber in BORON NEUTRON CAPTURE THERAPY. Boron-11,Boron 11
D002626 Chemistry, Pharmaceutical Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use. Medicinal Chemistry,Chemistry, Pharmaceutic,Pharmaceutic Chemistry,Pharmaceutical Chemistry,Chemistry, Medicinal
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004364 Pharmaceutical Preparations Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form. Drug,Drugs,Pharmaceutical,Pharmaceutical Preparation,Pharmaceutical Product,Pharmaceutic Preparations,Pharmaceutical Products,Pharmaceuticals,Preparations, Pharmaceutical,Preparation, Pharmaceutical,Preparations, Pharmaceutic,Product, Pharmaceutical,Products, Pharmaceutical

Related Publications

David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
January 2018, Current medicinal chemistry,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
January 2024, Journal of pharmaceutical and biomedical analysis,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
April 1992, Journal of chromatography,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
September 2001, Electrophoresis,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
January 2010, Advances in chromatography,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
May 2018, Gels (Basel, Switzerland),
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
April 2008, Journal of chromatography. A,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
March 2023, Journal of nanobiotechnology,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
January 2018, Current pharmaceutical design,
David S Hage, and Jeanethe A Anguizola, and Cong Bi, and Rong Li, and Ryan Matsuda, and Efthimia Papastavros, and Erika Pfaunmiller, and John Vargas, and Xiwei Zheng
July 1964, Produits & problemes pharmaceutiques,
Copied contents to your clipboard!