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Monoclonal antibody CMA134.1 reacted with a protein antigen of apparent molecular mass 22 kDa from Mycobacterium bovis and Mycobacterium tuberculosis, and with an apparently 24 kDa antigen of Mycobacterium kansasii, but not with other mycobacteria or related species. This antibody was used to screen a gene library of M. bovis in lambda gt11 and identified a recombinant clone that expressed a protein with an apparent molecular mass of 19-20 kDa. Gene expression occurred from the lac promoter in lambda gt11, but used an unidentified vector promoter, possibly that of the replication primer RNA, in the final plasmid construct. The sequence of an 840 bp fragment was determined and shown to code for a product of 15 kDa. This sequence is identical to that, independently determined, of a gene from M. tuberculosis, usually referred to as the 19 kDa antigen. The reasons for the apparent size discrepancies are discussed.
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
September 1988,
Journal of bacteriology,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
January 1989,
Infection and immunity,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
May 1987,
Microbial pathogenesis,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
April 1989,
Journal of general microbiology,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
November 1997,
Microbiology (Reading, England),
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
December 1996,
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
February 1989,
Nucleic acids research,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
July 1990,
Nucleic acids research,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
November 2002,
Preparative biochemistry & biotechnology,
M E Collins, and
A Patki, and
S Wall, and
A Nolan, and
J Goodger, and
M J Woodward, and
J W Dale
April 1988,
Infection and immunity,
