BIOMAT Database Logo BIOMAT Database Logo

Restoration of critical-size defects in the rabbit mandible using porous nanohydroxyapatite-polyamide scaffolds. 2012

Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, P.R. China.

Composite nanohydroxyapatite/polyamide (n-HA/PA) biomaterials have been indicated for bone defect reconstruction, where PA is added to enhance the toughness of n-HA. However, a comprehensive understanding of the biological performance of this implant material remains to be determined. In this study, the biological activity of n-HA/PA biomaterials was characterized in vitro by assessing the growth of bone marrow stromal cells (BMSCs), and in an in vivo rabbit model. To evaluate the n-HA/PA performance under different osteogenic conditions in vivo, implants were inserted to critical-size bone defects in the angle and body of the rabbit mandible. To determine the necessity of ectogenic BMSC-n-HA/PA hybrids at different implantation sites, both raw n-HA/PA materials and BMSC-seeded n-HA/PA hybrids were implanted. Bone formation was detected by radiology and histological studies. The results showed that n-HA/PA composites had great bioactivity, demonstrating significant BMSC proliferation, active alkaline phosphatase secretion, and stimulating the expression of osteogenic proteins (bone morphogenetic protein 2 [BMP2], osteoprotegerin [OPG], osteopontin [OPN], collagen type I [Col I], and osteocalcin [OCN]), in comparison to the control (polyethylene). At marrow-rich implantation sites (mandibular body), the amount of new bone formation was significant, but was not enhanced by the presence of BMSCs in the BMSC-n-HA/PA hybrids. However, the BMSC-n-HA/PA hybrids were essential for promoting bone formation in marrow-poor sites (mandibular angle). In conclusion, n-HA/PA biomaterials, which offer the advantage of enhanced mechanical performance over n-HA, exhibit significant bioactivity, including the capacity for bone regeneration at marrow-poor sites when implanted in combination with BMSCs.

UI MeSH Term Description Entries
D008334 Mandible The largest and strongest bone of the FACE constituting the lower jaw. It supports the lower teeth. Mylohyoid Groove,Mylohyoid Ridge,Groove, Mylohyoid,Grooves, Mylohyoid,Mandibles,Mylohyoid Grooves,Mylohyoid Ridges,Ridge, Mylohyoid,Ridges, Mylohyoid
D008422 Materials Testing The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility. Biocompatibility Testing,Biocompatible Materials Testing,Hemocompatibility Testing,Testing, Biocompatible Materials,Testing, Hemocompatible Materials,Hemocompatibility Testings,Hemocompatible Materials Testing,Materials Testing, Biocompatible,Materials Testing, Hemocompatible,Testing, Biocompatibility,Testing, Hemocompatibility,Testing, Materials,Testings, Biocompatibility
D009757 Nylons Polymers where the main polymer chain comprises recurring amide groups. These compounds are generally formed from combinations of diamines, diacids, and amino acids and yield fibers, sheeting, or extruded forms used in textiles, gels, filters, sutures, contact lenses, and other biomaterials. Polyamides,Dermalon,Ethilon,Nylon,Polyamide,Supramid,Dermalons,Ethilons,Supramids
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D001861 Bone Regeneration Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone. Osteoconduction,Bone Regenerations,Regeneration, Bone,Regenerations, Bone
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000469 Alkaline Phosphatase An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001672 Biocompatible Materials Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function. Biomaterials,Bioartificial Materials,Hemocompatible Materials,Bioartificial Material,Biocompatible Material,Biomaterial,Hemocompatible Material,Material, Bioartificial,Material, Biocompatible,Material, Hemocompatible

Related Publications

Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect.
April 2017, ACS applied materials & interfaces,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
The repair of critical-size defects with porous hydroxyapatite/polyamide nanocomposite: an experimental study in rabbit mandibles.
May 2010, International journal of oral and maxillofacial surgery,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Are critical size bone notch defects possible in the rabbit mandible?
April 2019, Journal of the Korean Association of Oral and Maxillofacial Surgeons,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Restoration of critical defects in the rabbit mandible using osteoblasts and vascular endothelial cells co-cultured with vascular stent-loaded nano-composite scaffolds.
December 2021, Journal of the mechanical behavior of biomedical materials,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds.
September 2014, Materials science & engineering. C, Materials for biological applications,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Tissue engineering scaffold material of porous nanohydroxyapatite/polyamide 66.
May 2010, International journal of nanomedicine,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Additively manufactured pure zinc porous scaffolds for critical-sized bone defects of rabbit femur.
January 2023, Bioactive materials,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Selective laser melting-produced porous titanium scaffolds regenerate bone in critical size cortical bone defects.
May 2013, Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Porous titanium granules in critical size defects of rabbit tibia with or without membranes.
June 2014, International journal of oral science,
Jun Guo, and Zhaosong Meng, and Gang Chen, and Dan Xie, and Yali Chen, and Hang Wang, and Wei Tang, and Lei Liu, and Wei Jing, and Jie Long, and Weihua Guo, and Weidong Tian
Critical-sized marginal defects around implants in the rabbit mandible.
April 2024, Oral and maxillofacial surgery,
Copied contents to your clipboard!