Biomaterial Database

Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains. 2012

Shinji Yamamoto, and Katsunori Suzuki

Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739–8526, Japan. syamamo@hiroshima-u.ac.jp

For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.

UI	MeSH Term	Description	Entries
D001427	Bacterial Toxins	Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.	Bacterial Toxin,Toxins, Bacterial,Toxin, Bacterial
D012641	Selection, Genetic	Differential and non-random reproduction of different genotypes, operating to alter the gene frequencies within a population.	Natural Selection,Genetic Selection,Selection, Natural
D017384	Sequence Deletion	Deletion of sequences of nucleic acids from the genetic material of an individual.	Deletion Mutation,Deletion Mutations,Deletion, Sequence,Deletions, Sequence,Mutation, Deletion,Mutations, Deletion,Sequence Deletions
D042822	Genomic Instability	An increased tendency of the GENOME to acquire MUTATIONS when various processes involved in maintaining and replicating the genome are dysfunctional.	Genome Instability,Genome Stability,Genomic Stability,Genome Instabilities,Genome Stabilities,Genomic Instabilities,Genomic Stabilities,Instabilities, Genome,Instabilities, Genomic,Instability, Genome,Instability, Genomic,Stabilities, Genome,Stabilities, Genomic,Stability, Genome,Stability, Genomic
D050296	Microbial Viability	Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.	Bacterial Viability,Virus Viability,Bacteria Viability,Microbial Inactivation,Inactivation, Microbial,Viability, Bacteria,Viability, Bacterial,Viability, Microbial,Viability, Virus
D060054	Agrobacterium	A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.
D036741	Plant Tumor-Inducing Plasmids	Plasmids coding for proteins which induce PLANT TUMORS. The most notable example of a plant tumor inducing plasmid is the Ti plasmid found associated with AGROBACTERIUM TUMEFACIENS.	Plant Tumor-Inducing Plasmid,Ti Plasmid,Ti Plasmid, Agrobacterium tumefaciens,Ti Plasmids,Plant Tumor Inducing Plasmid,Plant Tumor Inducing Plasmids,Plasmid, Plant Tumor-Inducing,Plasmid, Ti,Plasmids, Plant Tumor-Inducing,Plasmids, Ti,Tumor-Inducing Plasmid, Plant,Tumor-Inducing Plasmids, Plant