Biomaterial Database

Single-strand DNA breaks in human hair root cells exposed to mobile phone radiation. 2012

Semra Tepe Çam, and Nesrin Seyhan

Seyhan Gazi University Faculty of Medicine Biophysics Department, Besevler, Ankara, Turkey. stepe06@gmail.com

OBJECTIVE To analyze the short-term effects of radiofrequency radiation (RFR) exposure on genomic deoxyribonucleic acid (DNA) of human hair root cells. METHODS Hair samples were collected from eight healthy human subjects immediately before and after using a 900-MHz GSM (Global System for Mobile Communications) mobile phone for 15 and 30 min. Single-strand DNA breaks of hair root cells from the samples were determined using the 'comet assay'. RESULTS The data showed that talking on a mobile phone for 15 or 30 min significantly increased (p < 0.05) single-strand DNA breaks in cells of hair roots close to the phone. Comparing the 15-min and 30-min data using the paired t-test also showed that significantly more damages resulted after 30 min than after 15 min of phone use. CONCLUSIONS A short-term exposure (15 and 30 min) to RFR (900-MHz) from a mobile phone caused a significant increase in DNA single-strand breaks in human hair root cells located around the ear which is used for the phone calls.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D004781	Environmental Exposure	The exposure to potentially harmful chemical, physical, or biological agents in the environment or to environmental factors that may include ionizing radiation, pathogenic organisms, or toxic chemicals.	Exposure, Environmental,Environmental Exposures,Exposures, Environmental
D005260	Female		Females
D006197	Hair	A filament-like structure consisting of a shaft which projects to the surface of the SKIN from a root which is softer than the shaft and lodges in the cavity of a HAIR FOLLICLE. It is found on most surfaces of the body.	Fetal Hair,Hair, Fetal,Lanugo,Fetal Hairs,Hairs,Hairs, Fetal
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D053904	DNA Breaks, Single-Stranded	Interruptions in one of the strands of the sugar-phosphate backbone of double-stranded DNA.	Single-Stranded DNA Breaks,DNA Nicks,Single-Strand DNA Breaks,Single-Stranded DNA Break,Break, Single-Strand DNA,Break, Single-Stranded DNA,Breaks, Single-Strand DNA,Breaks, Single-Stranded DNA,DNA Break, Single-Strand,DNA Break, Single-Stranded,DNA Breaks, Single Stranded,DNA Breaks, Single-Strand,DNA Nick,Nick, DNA,Nicks, DNA,Single Strand DNA Breaks,Single Stranded DNA Break,Single Stranded DNA Breaks,Single-Strand DNA Break
D020552	Comet Assay	A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.	Alkaline Comet Assay,Alkaline Single-Cell Gel Electrophoresis Assay,Electrophoresis, Gel, Single-Cell,Gel Electrophoresis, Single-Cell,Alkaline Comet Assays,Alkaline Single Cell Gel Electrophoresis Assay,Assay, Alkaline Comet,Assay, Comet,Assays, Alkaline Comet,Assays, Comet,Comet Assay, Alkaline,Comet Assays,Comet Assays, Alkaline,Electrophoreses, Single-Cell Gel,Electrophoresis, Single-Cell Gel,Gel Electrophoreses, Single-Cell,Gel Electrophoresis, Single Cell,Single-Cell Gel Electrophoreses,Single-Cell Gel Electrophoresis
D040421	Cell Phone	Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.	Car Phone,Cell Phones,Cellular Phone,Mobile Phone,Telephone, Cellular,Mobile Telephone,Portable Cellular Phone,Transportable Cellular Phone,Car Phones,Cellular Phone, Portable,Cellular Phone, Transportable,Cellular Phones,Cellular Phones, Portable,Cellular Phones, Transportable,Cellular Telephone,Cellular Telephones,Mobile Phones,Mobile Telephones,Phone, Car,Phone, Cell,Phone, Cellular,Phone, Mobile,Phones, Car,Phones, Cell,Phones, Cellular,Phones, Mobile,Portable Cellular Phones,Telephone, Mobile,Telephones, Cellular,Telephones, Mobile,Transportable Cellular Phones