Biomaterial Database

Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans. 2012

Yasuaki Saitoh, and Masumi Katane, and Tomonori Kawata, and Kazuhiro Maeda, and Masae Sekine, and Takemitsu Furuchi, and Hiroyuki Kobuna, and Taro Sakamoto, and Takao Inoue, and Hiroyuki Arai, and Yasuhito Nakagawa, and Hiroshi Homma

Laboratory of Biomolecular Science, Department of Pharmaceutical Life Sciences, Kitasato University, Minato-ku, Tokyo, Japan.

Recent investigations have shown that a variety of D-amino acids are present in living organisms and that they possibly play important roles in physiological functions in the body. D-Amino acid oxidase (DAO) and D-aspartate oxidase (DDO) are degradative enzymes stereospecific for D-amino acids. They have been identified in various organisms, including mammals and the nematode Caenorhabditis elegans, although the significance of these enzymes and the relevant functions of D-amino acids remain to be elucidated. In this study, we investigated the spatiotemporal localization of C. elegans DAO and DDOs (DDO-1, DDO-2, and DDO-3) and measured the levels of several D- and L-amino acids in wild-type C. elegans and four mutants in which each gene for DAO and the DDOs was partially deleted and thereby inactivated. Furthermore, several phenotypes of these mutant strains were characterized. The results reported in this study indicate that C. elegans DAO and DDOs are involved in egg-laying events and the early development of C. elegans. In particular, DDOs appear to play important roles in the development and maturation of germ cells. This work provides novel and useful insights into the physiological functions of these enzymes and D-amino acids in multicellular organisms.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D003605	D-Amino-Acid Oxidase		dextro-Amino Acid Oxidase,D-Amino Acid Dehydrogenase,Acid Dehydrogenase, D-Amino,Acid Oxidase, dextro-Amino,D Amino Acid Dehydrogenase,D Amino Acid Oxidase,Dehydrogenase, D-Amino Acid,Oxidase, D-Amino-Acid,Oxidase, dextro-Amino Acid,dextro Amino Acid Oxidase
D004625	Embryo, Nonmammalian	The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.	Embryonic Structures, Nonmammalian,Embryo, Non-Mammalian,Embryonic Structures, Non-Mammalian,Nonmammalian Embryo,Nonmammalian Embryo Structures,Nonmammalian Embryonic Structures,Embryo Structure, Nonmammalian,Embryo Structures, Nonmammalian,Embryo, Non Mammalian,Embryonic Structure, Non-Mammalian,Embryonic Structure, Nonmammalian,Embryonic Structures, Non Mammalian,Embryos, Non-Mammalian,Embryos, Nonmammalian,Non-Mammalian Embryo,Non-Mammalian Embryonic Structure,Non-Mammalian Embryonic Structures,Non-Mammalian Embryos,Nonmammalian Embryo Structure,Nonmammalian Embryonic Structure,Nonmammalian Embryos,Structure, Non-Mammalian Embryonic,Structure, Nonmammalian Embryo,Structure, Nonmammalian Embryonic,Structures, Non-Mammalian Embryonic,Structures, Nonmammalian Embryo,Structures, Nonmammalian Embryonic
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017173	Caenorhabditis elegans	A species of nematode that is widely used in biological, biochemical, and genetic studies.	Caenorhabditis elegan,elegan, Caenorhabditis
D050804	D-Aspartate Oxidase	An FAD-dependent peroxisomal flavoenzyme, this catalyzes the oxidative deamination of D-ASPARTATE to OXALOACETATE and AMMONIA using oxygen as electron acceptor.	Aspartic Oxidase,D-aspartic Oxidase,D Aspartate Oxidase,D aspartic Oxidase,Oxidase, Aspartic,Oxidase, D-Aspartate,Oxidase, D-aspartic
D052287	Oviparity	The capability of producing eggs (OVA) from which young are hatched outside the body. While mostly referring to nonmammalian species, this does include MAMMALS of the order MONOTREMATA.	Oviparous
D029742	Caenorhabditis elegans Proteins	Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.	C elegans Proteins