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Observations on the mechanism of the reversible epimerization of GDP-D-mannose to GDP-L-galactose by an enzyme from Chlorella pyrenoidosa. 1979

G A Barber

An enzyme fraction from the green alga Chlorella pyrenoidosa that catalyzes the reversible epimerization of guanosine 5'-diphosphate D-mannose to guanosine 5'-diphosphate L-galactose brings about the incorporation of tritium from tritium-labeled water into the hexosyl moieties of those sugar nucleotides. The hexoses were degraded by periodate oxidation whereby the tritium was found to be equally distributed between carbon atoms 3 and 5. That observation was taken to imply that the epimerizations proceed via ene-diol intermediates.

UI MeSH Term Description Entries
D009702 Nucleoside Diphosphate Sugars Diphosphate Sugars, Nucleoside,Sugars, Nucleoside Diphosphate
D010084 Oxidation-Reduction A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471). Redox,Oxidation Reduction
D010504 Periodic Acid A strong oxidizing agent. Paraperiodic Acid,Periodic Acid (HIO4),Periodic Acids,Acid, Paraperiodic,Acid, Periodic,Acids, Periodic
D002238 Carbohydrate Epimerases Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3. Carbohydrate Isomerases,Epimerases, Carbohydrate,Isomerases, Carbohydrate
D002708 Chlorella Nonmotile unicellular green algae potentially valuable as a source of high-grade protein and B-complex vitamins. Chlorellas
D005690 Galactose An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood. D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D006155 Guanosine Diphosphate Mannose A nucleoside diphosphate sugar which can be converted to the deoxy sugar GDPfucose, which provides fucose for lipopolysaccharides of bacterial cell walls. Also acts as mannose donor for glycolipid synthesis. GDP Mannose,Guanosine Diphosphomannose,Guanosine Pyrophosphate Mannose,Diphosphate Mannose, Guanosine,Diphosphomannose, Guanosine,Mannose, GDP,Mannose, Guanosine Diphosphate,Mannose, Guanosine Pyrophosphate,Pyrophosphate Mannose, Guanosine
D006156 Guanosine Diphosphate Sugars Esters formed between the aldehydic carbon of sugars and the terminal phosphate of guanosine diphosphate. GDP Sugars,Diphosphate Sugars, Guanosine,Sugars, GDP,Sugars, Guanosine Diphosphate

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Formation of GDP-L-galactose from GDP-D-mannose.
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[Study on the enzyme transfer of D-(14C) mannose from GDP-(14C) mannose, on an endogenous acceptor found in the microsomes of Aspergillus niger].
January 1971, European journal of biochemistry,
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Quaternary assembly and crystal structure of GDP-D-mannose 4,6 dehydratase from Paramecium bursaria Chlorella virus.
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Disappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa.
October 1970, The Biochemical journal,
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An assay for GDP-D-mannose-4,6-dehydratase.
January 1985, Analytical biochemistry,
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The metabolism of D-mannose- 14 C to polysaccharide in corn roots. Specific labeling of L-galactose, D-mannose, and L-fucose.
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Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae.
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Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose.
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Identification and characterization of GDP-d-mannose 4,6-dehydratase and GDP-l-fucose snthetase in a GDP-l-fucose biosynthetic gene cluster from Helicobacter pylori.
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Epimerization of D-glucose to L-galactose during the biosynthesis of a sulfated L-galactan in the ascidian tunic.
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