Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov. 2013

Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.

A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species Mycobacterium arupense, three sqvs were detected in the 16S rRNA gene, six sqvs in the hsp65 gene and 15 sqvs in the rpoB gene; in Mycobacterium senuense two sqvs were present in each of the three genetic regions; in Mycobacterium kumamotonense four, two and nine sqvs were found, respectively, and in M. terrae three, four and six sqvs were found, respectively. The inappropriate inclusion of Mycobacterium triviale within the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of M. terrae complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to Mycobacterium hiberniae. The type strain of this species, of which eight strains were investigated, is ATCC 27353(T) ( = DSM 45694(T)). A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to M. arupense with respect to the 16S rRNA gene, M. heraklionense sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432(T) ( = LMG 24735(T) = CECT 7509(T)). Mycobacterium longobardum sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the M. terrae complex, clearly divergent from any other species. The type strain is DSM 45394(T) ( = CCUG 58460(T)).

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009170 Nontuberculous Mycobacteria So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. abscessus, M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi. Atypical Mycobacteria,Mycobacteria, Atypical,Mycobacterium duvalii,Mycobacterium flavescens,Mycobacterium gilvum,Mycobacterium gordonae,Mycobacterium obuense,Mycobacterium szulgai,Mycobacterium terrae,Mycolicibacter terrae,Mycolicibacterium duvalii,Mycolicibacterium flavescens,Mycolicibacterium gilvum,Mycolicibacterium obuense,Tuberculoid Bacillus,Atypical Mycobacterium,Mycobacterium, Atypical,Non-Tuberculous Mycobacteria,Nontuberculous Mycobacterium
D009171 Mycolic Acids Long fatty acids found in the CELL WALLS of some ACTINOBACTERIA, including MYCOBACTERIUM TUBERCULOSIS. Mycolic Acid,Acid, Mycolic,Acids, Mycolic
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D012336 RNA, Ribosomal, 16S Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis. 16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

Related Publications

Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
February 2015, International journal of systematic and evolutionary microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
October 2015, Experimental and therapeutic medicine,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
May 2016, Journal of clinical microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
March 2017, Journal of clinical microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
March 2008, International journal of systematic and evolutionary microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
November 2005, International journal of systematic and evolutionary microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
November 2009, International journal of systematic and evolutionary microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
January 2022, Mycobiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
September 2004, International journal of systematic and evolutionary microbiology,
Enrico Tortoli, and Zoe Gitti, and Hans-Peter Klenk, and Stefania Lauria, and Roberta Mannino, and Paola Mantegani, and Alessandro Mariottini, and Ioannis Neonakis
January 2008, International journal of systematic and evolutionary microbiology,
Copied contents to your clipboard!