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The possible involvement of Na+,K+-ATPase in the etiology of obesity in the obese (ob/ob) mouse was explored. The number of Na+,K+-ATPase enzyme units in skeletal muscle, liver, and kidneys from 4- and 8-wk-old obese and lean mice was estimated from saturable [3H]ouabain binding to particulate fractions. Neither phenotype nor age altered the Kd value for ouabain binding in these three tissue preparations. The total number of [3H]ouabain binding sites in hindlimb muscles was 35--55% lower in 4- and 8-wk-old obese mice than in their lean counterparts. However, the total number of [3H]ouabain binding sites in liver and kidneys of obese mice was similar to values observed in their lean counterparts. Because it has been suggested that ob/ob mice are hypothyroid, we investigated the response of Na+,K+-ATPase in these mice to thyroid hormone treatment (approximately 5 microgram thyroxine/day for 2 wk). The number of [3H]ouabain binding sites in the three tissues increased in both obese and lean mice injected with this relatively large dose of thyroxine, but the obese mice were 2--3 times more responsive than lean mice.
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
July 1979,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
May 1981,
Metabolism: clinical and experimental,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
January 1978,
Biochemical and biophysical research communications,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
July 1984,
Metabolism: clinical and experimental,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
July 2001,
Pancreas,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
February 1979,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
March 1983,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
April 1987,
Pharmacology, biochemistry, and behavior,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
July 1983,
The Biochemical journal,
M H Lin, and
J G Vander Tuig, and
D R Romsos, and
T Akera, and
G A Leveille
May 1986,
Experientia,
