Modulation of hepatitis C virus release by the interferon-induced protein BST-2/tetherin. 2012

Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
Department of Virology and Developmental Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, Israel.

Hepatitis C virus is a leading cause of chronic hepatitis and liver cancer. Little information exists on the interplay between innate defense mechanisms and viral antagonists that promote viral egress. Herein, the effects of Tetherin/BST-2 on HCV release were investigated. In Huh-7.5 hepatocytes, low expression levels of BST-2 were detected. Treatment of Huh-7.5 cells with IFNα, elevated BST-2 expression levels. However, HCV could not alter the expression of IFNα-induced BST-2, nor of stably over-expressed BST-2. Significantly, over expressed BST-2 moderately blocked HCV production and release from Huh-7.5 cells. Functional analysis of BST-2, confirmed its ability to inhibit the release of HIV delta-Vpu from Huh-7.5-BST-2 cells. HIV-Vpu antagonized BST-2 activity and rescued HIV delta-Vpu release from Huh-7.5-BST-2 cells. However, vpu slightly rescued HCV release and production from Huh-7.5-BST-2. We conclude that BST-2 moderately restricts HCV production and release from Huh-7.5 hepatocytes, while the virus lacks mechanisms to counteract this restriction.

UI MeSH Term Description Entries
D006526 Hepatitis C INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown. Hepatitis, Viral, Non-A, Non-B, Parenterally-Transmitted,Parenterally-Transmitted Non-A, Non-B Hepatitis,PT-NANBH,Parenterally Transmitted Non A, Non B Hepatitis
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015703 Antigens, CD Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation. CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation
D016174 Hepacivirus A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species. Hepatitis C virus,Hepatitis C-Like Viruses,Hepaciviruses,Hepatitis C Like Viruses,Hepatitis C viruses,Hepatitis C-Like Virus
D016898 Interferon-alpha One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES, and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways. Interferon Alfa,Interferon, Leukocyte,Interferon, Lymphoblast,alpha-Interferon,IFN-alpha D,IFN-alpha5,Interferon alpha-1,Interferon alpha-17,Interferon alpha-4,Interferon alpha-5,Interferon alpha-7,Interferon alpha-88,Interferon alpha-J,Interferon alpha-T,Interferon alpha4,Interferon alpha5,Interferon, Lymphoblastoid,Interferon, alpha,LeIF I,LeIF J,Leif D,IFN alpha D,IFN alpha5,Interferon alpha,Interferon alpha 1,Interferon alpha 17,Interferon alpha 4,Interferon alpha 5,Interferon alpha 7,Interferon alpha 88,Interferon alpha J,Interferon alpha T,Leukocyte Interferon,Lymphoblast Interferon,Lymphoblastoid Interferon,alpha Interferon
D057074 Virus Release Release of a virus from the host cell following VIRUS ASSEMBLY and maturation. Egress can occur by host cell lysis, EXOCYTOSIS, or budding through the plasma membrane. Virus Budding,Virus Egress,Budding, Virus,Egress, Virus,Release, Virus
D058851 GPI-Linked Proteins A subclass of lipid-linked proteins that contain a GLYCOSYLPHOSPHATIDYLINOSITOL LINKAGE which holds them to the CELL MEMBRANE. GPI-Anchored Proteins,GPI-Linked Membrane Proteins,Glycosylphosphatidylinositol Linked Proteins,GPI Anchored Proteins,GPI Linked Membrane Proteins,GPI Linked Proteins,Membrane Proteins, GPI-Linked,Proteins, GPI-Anchored,Proteins, GPI-Linked,Proteins, GPI-Linked Membrane,Proteins, Glycosylphosphatidylinositol Linked
D022781 Hepatocytes The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules. Hepatic Cells,Cell, Hepatic,Cells, Hepatic,Hepatic Cell,Hepatocyte

Related Publications

Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
December 2009, AIDS research and human retroviruses,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
June 2015, Scientific reports,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
May 2011, Viruses,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
June 2013, The Journal of general virology,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
June 2012, The Journal of biological chemistry,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
January 2012, Molecular biology international,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
March 2010, PLoS pathogens,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
June 2012, Current HIV research,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
December 2011, DNA and cell biology,
Avis Dafa-Berger, and Alona Kuzmina, and Michael Fassler, and Hila Yitzhak-Asraf, and Yonat Shemer-Avni, and Ran Taube
October 2010, Journal of virology,
Copied contents to your clipboard!