Increased transcription in hydroxyurea-treated root meristem cells of Vicia faba. 2013

Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
Department of Cytophysiology, Institute of Physiology, Cytology and Cytogenetics, University of Lodz, ul. Pomorska 141/143, 90-236 Łódź, Poland. winnicki@biol.uni.lodz.pl

Hydroxyurea (HU), an inhibitor of ribonucleotide reductase, prevents cells from progressing through S phase by depletion of deoxyribonucleoside triphosphates. Concurrently, disruption of DNA replication leads to double-strand DNA breaks. In root meristems of Vicia faba, HU triggers cell cycle arrest (preferentially in G1/S phase) and changes an overall metabolism by global activation of transcription both in the nucleoplasmic and nucleolar regions. High level of transcription is accompanied by an increase in the content of RNA polymerase II large subunit (POLR2A). Changes in transcription activation and POLR2A content correlate with posttranslational modifications of histones that play a role in opening up chromatin for transcription. Increase in the level of H4 Lys5 acetylation indicates that global activation of transcription following HU treatment depends on histone modifications.

UI MeSH Term Description Entries
D006918 Hydroxyurea An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxycarbamid,Hydrea,Oncocarbide
D000107 Acetylation Formation of an acetyl derivative. (Stedman, 25th ed) Acetylations
D012319 RNA Polymerase II A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6. DNA-Dependent RNA Polymerase II,RNA Pol II,RNA Polymerase B,DNA Dependent RNA Polymerase II
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D016193 G1 Phase The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors. First Gap Phase,G1a Phase,G1b Phase,Gap Phase 1,First Gap Phases,G1 Phases,G1a Phases,G1b Phases,Gap Phase, First,Gap Phases, First,Phase 1, Gap,Phase, First Gap,Phase, G1,Phase, G1a,Phase, G1b,Phases, First Gap,Phases, G1,Phases, G1a,Phases, G1b
D016196 S Phase Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome. S Period,Period, S,Periods, S,Phase, S,Phases, S,S Periods,S Phases
D059447 Cell Cycle Checkpoints Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable. Cell Cycle Arrest,Cell Cycle Control,Cell Cycle Transition Points,Cell Cycle-Transition Points,Arrest, Cell Cycle,Arrests, Cell Cycle,Cell Cycle Arrests,Cell Cycle Checkpoint,Cell Cycle Controls,Cell Cycle-Transition Point,Checkpoint, Cell Cycle,Checkpoints, Cell Cycle,Control, Cell Cycle,Controls, Cell Cycle,Cycle-Transition Point, Cell,Point, Cell Cycle-Transition
D018519 Meristem A group of plant cells that are capable of dividing infinitely and whose main function is the production of new growth at the growing tip of a root or stem. (From Concise Dictionary of Biology, 1990) Root Tip,Meristems,Root Tips,Tip, Root,Tips, Root
D031307 Vicia faba A plant species of the genus VICIA, family FABACEAE. The edible beans are well known but they cause FAVISM in some individuals with GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY. This plant contains vicine, convicine, Vicia lectins, unknown seed protein, AAP2 transport protein, and Vicia faba DNA-binding protein 1. Bean, Faba,Bean, Horse,Faba Bean,Fava Bean,Horsebean,Bean, Fava,Beans, Faba,Beans, Fava,Beans, Horse,Faba Beans,Fava Beans,Horse Bean,Horse Beans,Horsebeans,Vicia fabas,fabas, Vicia

Related Publications

Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
March 2024, Plant cell reports,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
September 1972, Indian journal of biochemistry & biophysics,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
January 1972, International journal of radiation biology and related studies in physics, chemistry, and medicine,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
October 2013, Protoplasma,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
May 1964, Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
June 1960, Experimental cell research,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
January 2012, Protoplasma,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
January 1977, Acta poloniae pharmaceutica,
Konrad Winnicki, and Justyna Teresa Polit, and Janusz Maszewski
December 1968, Radiation research,
Copied contents to your clipboard!