BIOMAT Database Logo BIOMAT Database Logo

Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation. 2012

Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, United Kingdom. Paul.bartley@moredun.ac.uk.

The immune responses of pregnant cattle and their foetuses were examined following inoculation on day 70 of gestation either intravenously (iv) (group 1) or subcutaneously (sc) (group 2) with live NC1 strain tachyzoites or with Vero cells (control) (group 3). Peripheral blood mononuclear cell (PBMC) responses to Neospora antigen and foetal viability were assessed throughout the experiment. Two animals from each group were sacrificed at 14, 28, 42 and 56 days post inoculation (pi). At post mortem, maternal lymph nodes, spleen and PBMC and when possible foetal spleen, thymus and PBMC samples were collected for analysis. Inoculation with NC1 (iv and sc) lead to foetal deaths in all group 1 dams (6/6) and in 3/6 group 2 dams from day 28pi; statistically significant (p ≤ 0.05) increases in cell-mediated immune (CMI) responses including antigen-specific cell proliferation and IFN-γ production as well as increased levels of IL-4, IL-10 and IL-12 were observed in challenged dams compared to the group 3 animals. Lymph node samples from the group 2 animals carrying live foetuses showed greater levels of cellular proliferation as well as significantly (p ≤ 0.05) higher levels of IFN-γ compared to the dams in group 2 carrying dead foetuses. Foetal spleen, thymus and PBMC samples demonstrated cellular proliferation as well as IFN-γ, IL-4, IL-10 and IL-12 production following mitogenic stimulation with Con A from day 14pi (day 84 gestation) onwards. This study shows that the generation of robust peripheral and local maternal CMI responses (lymphoproliferation, IFN-γ) may inhibit the vertical transmission of the parasite.

UI MeSH Term Description Entries
D007111 Immunity, Cellular Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role. Cell-Mediated Immunity,Cellular Immune Response,Cell Mediated Immunity,Cell-Mediated Immunities,Cellular Immune Responses,Cellular Immunities,Cellular Immunity,Immune Response, Cellular,Immune Responses, Cellular,Immunities, Cell-Mediated,Immunities, Cellular,Immunity, Cell-Mediated,Response, Cellular Immune
D007275 Injections, Intravenous Injections made into a vein for therapeutic or experimental purposes. Intravenous Injections,Injection, Intravenous,Intravenous Injection
D007279 Injections, Subcutaneous Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin. Subcutaneous Injections,Injection, Subcutaneous,Subcutaneous Injection
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418 Cattle Diseases Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus. Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D003048 Coccidiosis Protozoan infection found in animals and man. It is caused by several different genera of COCCIDIA. Besnoitiasis,Besnoitiosis,Besnoitiases,Besnoitioses,Coccidioses

Related Publications

Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation.
October 2013, Veterinary research,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation.
December 2012, Veterinary research,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation.
January 2021, Frontiers in cellular and infection microbiology,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Immune responses in pregnant cattle and bovine fetuses following experimental infection with Neospora caninum.
October 2001, Parasitology research,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Peripheral immune responses in pregnant cattle following Neospora caninum infection.
April 2007, Parasite immunology,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Maternal and foetal cytokine production in dams naturally and experimentally infected with Neospora caninum on gestation day 110.
August 2016, Research in veterinary science,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Experimental infection with a low virulence isolate of Neospora caninum at 70 days gestation in cattle did not result in foetopathy.
January 2009, Veterinary research,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Cellular immune responses in cattle experimentally infected with Neospora caninum.
November 1998, Parasite immunology,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
July 2013, Veterinary research,
Paul M Bartley, and Stephen E Wright, and Stephen W Maley, and Colin N Macaldowie, and Mintu Nath, and Clare M Hamilton, and Frank Katzer, and David Buxton, and Elisabeth A Innes
Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
January 2016, Veterinary research,
Copied contents to your clipboard!