Vitamin D receptor upregulation in alloreactive human T cells. 2012

Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
Division of Hematology and Oncology, Mayo Clinic, Jacksonville, FL, USA.

Vitamin D deficiency is adversely associated with diseases characterized by inflammation. The combination of the high incidence of vitamin D deficiency in patients undergoing allogeneic stem cell transplants (SCT) and the potential role of vitamin D deficiency in influencing graft-versus-host disease led us to further characterize the expression of VDR on alloreactive T cells. We hypothesized that vitamin D receptor expression may directly regulate alloreactive T cell responses. To overcome existing limitations in measuring VDR in bulk cellular populations, we developed a flow cytometric assay to measure cytoplasmic VDR in human T cells. Upon stimulation, VDR was expressed extremely early and exhibited sustained upregulation with chronic stimulation. VDR expression was also coupled to cytokine production, proliferation, and ERK1/2 phosphorylation. In addition, VDR exhibited a maturation stage-specific pattern of expression, with greatest expression on cells known to mediate GVHD, naïve and early memory T cells. Alloreactive T cells upregulated VDR, whereas the nonreactive T cells did not. Finally, repletion of vitamin D in vitro was sufficient to significantly reduce alloreactive T cell responses. These data suggest that vitamin D effects on T cells may be important in reducing graft versus host disease (GVHD) in the allogeneic stem cell transplant setting.

UI MeSH Term Description Entries
D007156 Immunologic Memory The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus. Immune Memory,Immunological Memory,Memory, Immunologic,Immune Memories,Immunologic Memories,Immunological Memories,Memory, Immune,Memory, Immunological
D007519 Isoantigens Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS. Alloantigens,Alloantigen,Isoantigen
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006086 Graft vs Host Disease The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION. Graft-Versus-Host Disease,Homologous Wasting Disease,Runt Disease,Graft-vs-Host Disease,Disease, Graft-Versus-Host,Disease, Graft-vs-Host,Disease, Homologous Wasting,Disease, Runt,Diseases, Graft-Versus-Host,Diseases, Graft-vs-Host,Graft Versus Host Disease,Graft-Versus-Host Diseases,Graft-vs-Host Diseases
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D014807 Vitamin D A vitamin that includes both CHOLECALCIFEROLS and ERGOCALCIFEROLS, which have the common effect of preventing or curing RICKETS in animals. It can also be viewed as a hormone since it can be formed in SKIN by action of ULTRAVIOLET RAYS upon the precursors, 7-dehydrocholesterol and ERGOSTEROL, and acts on VITAMIN D RECEPTORS to regulate CALCIUM in opposition to PARATHYROID HORMONE.

Related Publications

Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
January 1987, Methods in enzymology,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
April 2010, Nature immunology,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
January 2014, PloS one,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
December 1990, Scandinavian journal of immunology,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
January 2011, Annals of the New York Academy of Sciences,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
May 1997, Archives of dermatological research,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
April 1985, European journal of immunology,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
March 2002, Immunology,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
May 2014, Annals of the New York Academy of Sciences,
Richard W Joseph, and Ulas D Bayraktar, and Tae Kon Kim, and Lisa S St John, and Uday Popat, and Jahan Khalili, and Jeffrey J Molldrem, and Eric D Wieder, and Krishna V Komanduri
February 1987, Transplantation proceedings,
Copied contents to your clipboard!