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Crude preparations of kinetoplast vesicles were used to investigate the respiratory chain components in Leishmania tropica promastigotes. In difference spectra from enzymically and chemically reduced preparations, cytochrome b was the predominant component. By utilizing special assays designed to minimize the influence of cytochrome b on difference spectra, cytochromes a, a3 and c555 were demonstrated. Difference spectra from chemically reduced preparations indicated that pyridine nucleotides (NADH) and flavoproteins were also part of the respiratory chain. The presence of these components as well as their response to respiratory inhibitors and ascorbate provide evidence for the presence of a typical trypanosomatid respiratory chain in L. tropica promastigotes.
E Martin, and
A J Mukkada
February 1976,
Experimental parasitology,
E Martin, and
A J Mukkada
June 1977,
Experimental parasitology,
E Martin, and
A J Mukkada
January 1983,
Molecular and biochemical parasitology,
E Martin, and
A J Mukkada
May 1987,
Molecular and biochemical parasitology,
E Martin, and
A J Mukkada
December 1995,
Molecular and biochemical parasitology,
E Martin, and
A J Mukkada
September 1965,
Journal of the Philippine Medical Association,
E Martin, and
A J Mukkada
September 1983,
Molecular and biochemical parasitology,
E Martin, and
A J Mukkada
October 1986,
Molecular and biochemical parasitology,
E Martin, and
A J Mukkada
September 1978,
Tropenmedizin und Parasitologie,
E Martin, and
A J Mukkada
August 1976,
The Journal of protozoology,
