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We have recently described an intracellular protein, p21, in nonproducer cells transformed by either the Kirsten (Ki-MSV) or Harvey (Ha-MSV) strain of murine sarcoma virus (Shih et al., Virology, in press). The p21 is phosphorylated and has been shown to be coded for by either Ki-MSV or Ha-MSV. In this report, we compare the thermal stability of the newly synthesized [35S]methionine-labeled p21 in cells transformed by the wild-type Ki-MSV or by a mutant of Ki-MSV (ts 371) which is temperature sensitive in a viral function required for the maintenance of several properties of the transformed phenotype. The immunoprecipitability of the p21 coded for by the ts 371 Ki-MSV was markedly more thermolabile than the p21 of the wild-type Ki-MSV when the cell extracts are heated in vitro. The present finding suggests that the p21 is required for the maintenance of transformation induced by Ki-MSV.
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
November 1986,
Journal of virology,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
May 1984,
Journal of virology,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
August 1979,
Proceedings of the National Academy of Sciences of the United States of America,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
August 1973,
Proceedings of the National Academy of Sciences of the United States of America,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
November 1983,
Molecular and cellular biology,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
July 1981,
Cell,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
January 1976,
Cancer research,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
September 1981,
Proceedings of the National Academy of Sciences of the United States of America,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
April 1982,
Virology,
T Y Shih, and
M O Weeks, and
H A Young, and
E M Scolnick
December 1985,
Journal of virology,
