Comparative zymographic analysis of metallopeptidase of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis isolates from Peru. 2012

Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
Laboratório de Biologia Molecular de Tripanosomatídeos, Universidad Peruana Cayetano Heredia, Lima, Peru.

American tegumentary leishmaniasis (ATL) in Peru is mainly associated with Leishmania (Viannia) peruviana and L. (V.) braziliensis. These parasites are genetically related, and their characterization as distinct species is controversial. Despite their genetic similarity, each species is associated with different clinical manifestations of ATL; L. (V.) peruviana causes only cutaneous leishmaniasis, whereas L. (V.) braziliensis can cause both cutaneous and mucocutaneous leishmaniasis. Because the primary cutaneous lesions caused by infection with these species are indistinguishable, it is necessary to develop a suitable method to differentiate them in order to prevent possible metastasis to oropharyngeal mucosa. In the present study, we investigated the proteolytic profile of L. (V.) peruviana and L. (V.) braziliensis isolates from Peru by zymographic analysis in SDS-PAGE copolymerized with gelatin. Enzymes were characterized according to their pH range of activity and sensitivity to distinct peptidase inhibitors. We observed that L. (V.) peruviana isolates displayed three proteolytic bands with molecular masses ranging from 55 to 80 kDa, whereas L. (V.) braziliensis isolates showed six proteolytic activities between 55 and 130 kDa. Using specific inhibitors, we determined that these proteolytic activities are due to metallopeptidases and present optimal activity between the pH range 5.5 and 10.0. Our results suggest that the expression of metallopeptidases in L. (V.) peruviana and L. (V.) braziliensis isolates from Peru is species-specific.

UI MeSH Term Description Entries
D007891 Leishmania A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi. Leishmania (Leishmania),Leishmania (Viannia),Leishmania leishmania,Leishmania viannia,Leishmania leishmanias,Leishmania viannias,Leishmanias,Leishmanias (Leishmania),Leishmanias (Viannia),leishmanias, Leishmania,viannias, Leishmania
D010568 Peru A country in western South America, bordering the South Pacific Ocean, and located between Chile and Ecuador.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D015971 Gene Expression Regulation, Enzymologic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis. Enzymologic Gene Expression Regulation,Regulation of Gene Expression, Enzymologic,Regulation, Gene Expression, Enzymologic
D016773 Leishmaniasis, Cutaneous An endemic disease that is characterized by the development of single or multiple localized lesions on exposed areas of skin that typically ulcerate. The disease has been divided into Old and New World forms. Old World leishmaniasis is separated into three distinct types according to epidemiology and clinical manifestations and is caused by species of the L. tropica and L. aethiopica complexes as well as by species of the L. major genus. New World leishmaniasis, also called American leishmaniasis, occurs in South and Central America and is caused by species of the L. mexicana or L. braziliensis complexes. Leishmaniasis, American,Leishmaniasis, New World,Leishmaniasis, Old World,Oriental Sore,American Leishmaniasis,Cutaneous Leishmaniases,Cutaneous Leishmaniasis,Leishmaniases, Cutaneous,New World Leishmaniasis,Old World Leishmaniasis,Sore, Oriental
D045726 Metalloproteases Proteases which use a metal, normally ZINC, in the catalytic mechanism. This group of enzymes is inactivated by metal CHELATORS. Metallopeptidase,Metalloprotease,Metalloproteinase,Metalloproteinases,Metallopeptidases

Related Publications

Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
September 2015, BMC genomics,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
September 1995, Parasitology,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
January 1990, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
January 1999, Acta tropica,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
December 1995, Annals of tropical medicine and parasitology,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
December 2020, Revista peruana de medicina experimental y salud publica,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
January 2015, Revista peruana de medicina experimental y salud publica,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
February 2020, The American journal of tropical medicine and hygiene,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
January 2016, PLoS neglected tropical diseases,
Patrícia Reyes-Uribe, and Taissa Pereira-dos-Santos, and Jose B De Jesus, and Camila Mesquita-Rodrigues, and Jorge Arevalo, and Elisa Cupolillo, and Patricia Cuervo
February 2006, Parasitology,
Copied contents to your clipboard!