Simultaneous determination of amoxicillin and ampicillin in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection using pre-column derivatization. 2012

Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China.

A sensitive and robust method is presented for the simultaneous determination of amoxicillin (AMO) and ampicillin (AMP) in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection (RP-HPLC-FLD). This method used a simple liquid-liquid extraction of the samples with acetonitrile and dichloromethane as precipitation of proteins and extraction solvent. AMO and AMP reacted with salicylaldehyde to form fluorescent derivatives, which were then analyzed with RP-HPLC-FLD. Separation was carried out on an Athena C18 column with a mobile phase consisting of 0.01 M potassium dihydrogen phosphate, adjusted to pH 5.5 by 2M potassium hydroxide and acetonitrile. The detector response was linear over the tested concentration range from 5.0 to 800 ng/mL for AMO and AMP. The recovery values ranged from 78.4 to 88.7% for AMO and from 77.6 to 82.0% for AMP. The limits of detection were 1.2 for AMO and 0.4 µg/kg for AMP. The limits of quantification were 3.9 for AMO and 1.5 µg/kg for AMP. The corresponding intra-day and inter-day variation (relative standard deviation) were found to be less than 9.6 and 14.8%, respectively.

UI MeSH Term Description Entries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004350 Drug Residues Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment. Drug Residue,Residue, Drug,Residues, Drug
D004531 Eggs Animal reproductive bodies, or the contents thereof, used as food. The concept is differentiated from OVUM, the anatomic or physiologic entity.
D000447 Aldehydes Organic compounds containing a carbonyl group in the form -CHO. Aldehyde
D000658 Amoxicillin A broad-spectrum semisynthetic antibiotic similar to AMPICILLIN except that its resistance to gastric acid permits higher serum levels with oral administration. Hydroxyampicillin,Actimoxi,Amoxicillin Anhydrous,Amoxicillin Monopotassium Salt,Amoxicillin Monosodium Salt,Amoxicillin Sodium,Amoxicillin Trihydrate,Amoxicillin, (R*)-Isomer,Amoxicilline,Amoxil,Amoxycillin,BRL-2333,Clamoxyl,Clamoxyl G.A.,Clamoxyl Parenteral,Penamox,Polymox,Trimox,Wymox,BRL 2333,BRL2333
D000667 Ampicillin Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic. Penicillin, Aminobenzyl,Amcill,Aminobenzylpenicillin,Ampicillin Sodium,Ampicillin Trihydrate,Antibiotic KS-R1,Omnipen,Pentrexyl,Polycillin,Ukapen,Aminobenzyl Penicillin,Antibiotic KS R1,KS-R1, Antibiotic,Sodium, Ampicillin,Trihydrate, Ampicillin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016014 Linear Models Statistical models in which the value of a parameter for a given value of a factor is assumed to be equal to a + bx, where a and b are constants. The models predict a linear regression. Linear Regression,Log-Linear Models,Models, Linear,Linear Model,Linear Regressions,Log Linear Models,Log-Linear Model,Model, Linear,Model, Log-Linear,Models, Log-Linear,Regression, Linear,Regressions, Linear

Related Publications

Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
November 1997, Se pu = Chinese journal of chromatography,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
November 2009, Se pu = Chinese journal of chromatography,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
September 2008, Se pu = Chinese journal of chromatography,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
June 1996, Journal of chromatography. B, Biomedical applications,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
July 2003, Journal of chromatography. A,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
June 1999, Journal of chromatography. B, Biomedical sciences and applications,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
September 2014, Journal of chromatography. A,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
January 2015, Se pu = Chinese journal of chromatography,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
January 1985, Journal of chromatography,
Kaizhou Xie, and Longfei Jia, and Dong Xu, and Huisheng Guo, and Xing Xie, and Yuping Huang, and Xuesen Chen, and Wenbin Bao, and Guojun Dai, and Jinyu Wang
November 2007, Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition,
Copied contents to your clipboard!