Complete amino acid sequence of globin chains and biological activity of fragmented crocodile hemoglobin (Crocodylus siamensis). 2012

Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
Protein and Proteomics Research Group, Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.

Hemoglobin, α-chain, β-chain and fragmented hemoglobin of Crocodylus siamensis demonstrated both antibacterial and antioxidant activities. Antibacterial and antioxidant properties of the hemoglobin did not depend on the heme structure but could result from the compositions of amino acid residues and structures present in their primary structure. Furthermore, thirteen purified active peptides were obtained by RP-HPLC analyses, corresponding to fragments in the α-globin chain and the β-globin chain which are mostly located at the N-terminal and C-terminal parts. These active peptides operate on the bacterial cell membrane. The globin chains of Crocodylus siamensis showed similar amino acids to the sequences of Crocodylus niloticus. The novel amino acid substitutions of α-chain and β-chain are not associated with the heme binding site or the bicarbonate ion binding site, but could be important through their interactions with membranes of bacteria.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D002176 Candida albicans A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis). Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D006090 Gram-Negative Bacteria Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method. Gram Negative Bacteria
D006094 Gram-Positive Bacteria Bacteria which retain the crystal violet stain when treated by Gram's method. Gram Positive Bacteria
D006454 Hemoglobins The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements. Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous
D000489 Alligators and Crocodiles Large, long-tailed reptiles, including caimans, of the order Loricata. Caimans,Crocodiles,Alligators,Caymans,Alligator,Caiman,Cayman,Crocodile,Crocodiles and Alligators
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
November 2014, Protein expression and purification,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
June 2008, Molecular biology reports,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
March 2013, The protein journal,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
January 2017, Journal of microbiology and biotechnology,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
July 2012, Annals of clinical microbiology and antimicrobials,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
February 2018, The protein journal,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
May 2018, Biotechnology and applied biochemistry,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
March 1998, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
June 1995, General and comparative endocrinology,
Saowaluck Srihongthong, and Anawat Pakdeesuwan, and Sakda Daduang, and Tomohiro Araki, and Apisak Dhiravisit, and Sompong Thammasirirak
August 2016, The protein journal,
Copied contents to your clipboard!