OBJECTIVE To evaluate subfoveal choroidal thickness (CT) in eyes with normal-tension glaucoma (NTG) in patients who were visualized by enhanced depth imaging optical coherence tomography. METHODS In this observational, comparative case series, subfoveal CT was measured with enhanced depth imaging optical coherence tomography in 35 eyes of normal individuals and 32 eyes of patients with NTG. Subfoveal CT in each eye was analyzed by measuring the vertical distance from Bruch membrane to the innermost sclera layer. RESULTS The mean subfoveal CT in the normal individual group and the NTG patient group were 300.0±52.7 and 289.5±100.4 µm, respectively. There was no difference in subfoveal CT in the 2 groups (P=0.60). The mean subfoveal CT in all subjects was significantly correlated negatively with age only (P=0.03). CONCLUSIONS The subfoveal CT does not differ between the eyes of the normal individual group and the eyes in the NTG patient group, suggesting that CT may not play a role in the pathogenesis of NTG.