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For evaluation whether the membrane fatty acid-binding protein is related to mGOT, studies on the structure and function of both purified proteins were performed. Physicochemical characterization revealed that both proteins are different: the membrane fatty acid-binding protein has a molecular weight of 40 kD and a pI of 8.5-9.0, whereas rat mGOT has a molecular weight of 44 kD and a pI of 9.5-10.0. According to this distinct differences, they migrated separately on 2-dimensional electrophoresis. Furthermore, monospecific antibodies against the membrane fatty acid binding protein did not react with rat mGOT. In co-chromatography studies only the membrane fatty acid-binding protein showed affinity for long chain fatty acids, but not mGOT. Moreover, membrane binding studies were performed with the monospecific antibody to the membrane fatty acid binding protein. The inhibitory effect of this antibody on plasma membrane binding of oleate was reversed after preabsorption of the antibody with the membrane fatty acid binding protein, but was not affected after preabsorption with mGOT. These results indicate that the membrane fatty acid binding protein and mGOT are structurally and functionally not related. The data also support the significance of this membrane protein in the plasma membrane binding process of long chain fatty acids.
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
January 1990,
Annals of the New York Academy of Sciences,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
May 1990,
Proceedings of the National Academy of Sciences of the United States of America,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
March 1958,
British medical journal,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
July 1970,
Science (New York, N.Y.),
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
May 1979,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
October 1979,
Human genetics,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
September 1981,
Journal of biochemistry,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
September 1961,
Nordisk medicin,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
December 1956,
Canadian Medical Association journal,
W Stremmel, and
H E Diede, and
E Rodilla-Sala, and
K Vyska, and
M Schrader, and
B Fitscher, and
S Passarella
October 1973,
The Journal of the Association of Physicians of India,
