Development and diagnostic application/evaluation of pandemic (H1N1) 2009 influenza virus-specific monoclonal antibodies. 2012

Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
College of Pharmacy, Chungbuk National University, Chungbuk 361-763, Korea.

We prepared mAb specific to the H1N1 2009 virus (H1N1 2009) to facilitate development of an RDT with enhanced sensitivity and specificity. Among these antibodies, we identified two clones--hybridomas 1H7E1 and 3A3H7-that specifically bound to H1N1 2009 (non-seasonal) and were very suitable for application to a diagnostic kit. The affinity constants (K(a)) of 1H7E1 and 3A3H7 were 1.10 × 10(10) and 2.35 × 10(10), respectively. To identify the antibodies, we performed ELISA and immunoblot analyses and found that 1H7E1 recognized a conformational epitope of HA while 3A3H7 recognized a linear epitope. In clinical evaluations using specimens from 215 patients, a lateral flow rapid testing kit comprising these mAb showed a sensitivity of 81.5% (75/92) and a specificity of 96.7% (119/123). Results using the RDT kit were well correlated with conventional RT-PCR methods as commonly and commercially used. Based on our findings, we believe that use of these mAb with a rapid evaluation kit could serve as a good diagnostic tool for H1N1 2009.

UI MeSH Term Description Entries
D007251 Influenza, Human An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia. Grippe,Human Flu,Human Influenza,Influenza in Humans,Influenza,Flu, Human,Human Influenzas,Influenza in Human,Influenzas,Influenzas, Human
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D000915 Antibody Affinity A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes. Affinity, Antibody,Antibody Avidity,Avidity, Antibody,Affinities, Antibody,Antibody Affinities,Antibody Avidities,Avidities, Antibody
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity

Related Publications

Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
January 2011, Journal of clinical microbiology,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
March 2014, Archives of virology,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
July 2012, Journal of virological methods,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
March 2011, Clinical and vaccine immunology : CVI,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
April 2011, Journal of medical virology,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
December 2010, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
January 2014, Journal of virological methods,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
June 2017, Voprosy virusologii,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
January 2011, Voprosy virusologii,
Won-Shik Kim, and Gyu-Cheol Lee, and Jong-Ha Yoo, and Hak-Yong Kim, and Yeo-Pyo Yun, and Chom-Kyu Chong
October 2011, Chinese medical journal,
Copied contents to your clipboard!