-transparent.png){kind=logo}
Two techniques have been applied to the determination of the number and type (2-Fe, 4-Fe) of iron-sulfur centers in the iron-sulfur flavoprotein succinate dehydrogenase [succinate:(acceptor) oxidoreductase, EC 1.3.99.1]. One procedure uses p-CF3C6H4SH as an extrusion reagent and Fourier transform 19F nuclear magentic resonance as the method of detection and quantitation of extruded cores of these centers in the form of [Fe2S2(SRF)4]2- and [Fe4S4(SRF)4]2- (RF = p-C6H4CF3). The second procedure, interprotein core transfer, involves thiol displacement of iron-sulfur cores followed by specific core transfer to the apoproteins of Bacillus polymyxa ferredoxin and adrenodoxin. Detection and quantitation are accomplished by electron paramagnetic resonance of reduced proteins at low temperatures. Both procedures clearly show that succinate dehydrogenase contains two dimeric (Fe2S2) and one tetrameric (Fe4S4) centers per mole of histidyl flavin, accounting for all eight nonheme iron and eight labile sulfur atoms found by chemical analysis. These results remove uncertainties created by the less than stoichiometric amounts of binuclear centers detected by electron paramagnetic resonance after dithionite reduction and provide secure characterization of the iron-sulfur centers in this enzyme.
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
July 1977,
Archives of biochemistry and biophysics,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
July 1973,
Biochemical and biophysical research communications,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
January 1976,
Advances in experimental medicine and biology,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
May 1988,
FEBS letters,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
June 1990,
The Journal of biological chemistry,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
November 1994,
Gene,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
April 1999,
Biochimica et biophysica acta,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
March 1998,
Biochimica et biophysica acta,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
September 2006,
The Journal of biological chemistry,
C J Coles, and
R H Holm, and
D M Kurtz, and
W H Orme-Johnson, and
J Rawlings, and
T P Singer, and
G B Wong
January 1982,
Advances in experimental medicine and biology,
