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The Escherichia coli-Brevibacterium lactofermentum shuttle vector pBLA was introduced into intact cells of B. lactofermentum by electrotransformation. Several parameters of this procedure such as voltage and cell concentration were analysed. Optimal conditions gave an efficiency of 10(6) transformants per microgram of DNA. Two recalcitrant strains could be electrotransformed when an ampicillin pretreatment step was used. Electrotransformation experiments using DNAase or different structural forms of plasmid DNA showed that the electrotransformation process is quite different from natural transformation involving competence development. Restriction-modification-proficient B. lactofermentum could be efficiently electrotransformed with pBLA DNA isolated from E. coli. This restriction-modification system therefore seems to be overcome by electrotransformation. Thus electrotransformation may efficiently replace the protoplast bacterial transformation method.
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
April 1985,
Journal of bacteriology,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
February 1994,
Applied microbiology and biotechnology,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
June 1986,
Nucleic acids research,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
January 1994,
Microbiologia (Madrid, Spain),
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
November 2016,
Genetika,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
August 1991,
FEMS microbiology letters,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
June 1994,
FEMS microbiology letters,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
December 1997,
Letters in applied microbiology,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
January 1986,
Gene,
S Bonnassie, and
J F Burini, and
J Oreglia, and
A Trautwetter, and
J C Patte, and
A M Sicard
July 1996,
Plasmid,
