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Metaphase chromosomes isolated from a cell line carrying the thymidine kinase (TK) gene of herpes simplex virus type I were used to transform the TK-deficient cell line LMTK- to the TK+ phenotype. Four independent transformants were isolated, all of which expressed virus-specific TK. Each of the four transformant cell lines initially became TK- at a rate of 12% per day. All four transformants possessed multiple copies of the TK gene and in one of the four a rearrangement occurred adjacent to the TK sequences. Stable TK+ derivatives of each line, isolated after prolonged cultivation, retained fewer copies of the TK gene than did their unstable parents. The transferred chromosomal fragment was larger than 17 kilobases in each line.
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
November 1976,
In vitro,
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
January 1981,
Annual review of biochemistry,
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
January 1988,
Cancer surveys,
G A Scangos, and
K M Huttner, and
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F H Ruddle
January 1987,
Development (Cambridge, England),
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
January 1977,
Brookhaven symposia in biology,
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
November 1986,
Molecular and cellular biology,
G A Scangos, and
K M Huttner, and
S Silverstein, and
F H Ruddle
January 1980,
Annual review of genetics,
G A Scangos, and
K M Huttner, and
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F H Ruddle
January 1981,
Medicina,
G A Scangos, and
K M Huttner, and
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F H Ruddle
January 1979,
Developments in biological standardization,
G A Scangos, and
K M Huttner, and
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F H Ruddle
January 1998,
Anticancer research,
