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OBJECTIVE We examined the yield and quality of genomic deoxyribonucleic acid (DNA) extracted from various postmortem fetal tissues. METHODS Fetal tissues were collected at the time of autopsy, and DNA was subsequently extracted. The yield and DNA quality was assessed using ultraviolet spectrometry and agarose gel electrophoresis. We used polymerase chain reaction (PCR) to assess the DNA extracted for genomic testing. RESULTS The median (range) gestation of the fetuses was 22 (16-41) weeks and the postmortem interval was 5.5 (2-10) days. Non-degraded genomic DNA was successfully extracted from all fetal tissues. Liver tissue had the lowest quality and muscle the highest quality. DNA yield or purity was not influenced by the postmortem interval. CONCLUSIONS High quality genomic DNA can be extracted from fetal muscle, despite postmortem intervals of several days.
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March 2012,
Cold Spring Harbor protocols,
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November 2002,
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April 2003,
Analytical biochemistry,
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December 2012,
Genetics and molecular research : GMR,
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January 1994,
Journal - Forensic Science Society,
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October 2013,
Genetics and molecular research : GMR,
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Biomedical reports,
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January 2017,
Plant methods,
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January 2023,
PloS one,
