Moloney leukemia virus type 10 inhibits reverse transcription and retrotransposition of intracisternal a particles. 2012

Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
Department of Medicine, Rosalind Russell Medical Research Center, University of California at San Francisco (UCSF), San Francisco, California, USA.

Moloney leukemia virus type 10 protein (MOV10) is an RNA helicase that is induced by type I interferon. It inhibits HIV replication at several steps of its replicative cycle. Of interest, MOV10 is a component of mRNA processing (P) bodies, which inhibit retrotransposition (RTP) of intracisternal A particles (IAP). In this report, we studied the effects of MOV10 on IAP RTP and its dependence on P bodies. Indeed, MOV10 inhibited IAP RTP. It decreased significantly not only the products of reverse transcriptase but also its endogenous activity. MOV10 also associated with IAP RNA. Furthermore, although it was found in IAP virus-like particles, it did not affect their incorporation of IAP RNA, primer tRNAPhe (phenylalanine tRNA), or IAP Gag. Concerning P bodies, the exogenously expressed MOV10 had no effect on their size and number, and the inhibition of IAP RTP persisted despite the depletion of their RCK subunit. Thus, by interfering with reverse transcription, MOV10 inhibits IAP RTP, and this inhibition is independent of P bodies.

UI MeSH Term Description Entries
D008979 Moloney murine leukemia virus A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk. Moloney Leukemia Virus,Leukemia Virus, Moloney,Virus, Moloney Leukemia
D010649 Phenylalanine An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE. Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D003594 Cytoplasmic Granules Condensed areas of cellular material that may be bounded by a membrane. Cytoplasmic Granule,Granule, Cytoplasmic,Granules, Cytoplasmic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D012343 RNA, Transfer The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains. Suppressor Transfer RNA,Transfer RNA,tRNA,RNA, Transfer, Suppressor,Transfer RNA, Suppressor,RNA, Suppressor Transfer
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D047468 Immunoprecipitation The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution. Co-Immunoprecipitation,Immune Precipitation,Co Immunoprecipitation,Co-Immunoprecipitations,Immune Precipitations,Precipitation, Immune,Precipitations, Immune

Related Publications

Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
May 2010, The Journal of biological chemistry,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
November 1999, Journal of virology,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
July 2011, Journal of virology,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
April 1989, FEBS letters,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
September 2014, Bing du xue bao = Chinese journal of virology,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
June 2004, Virology,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
January 2010, Bioscience, biotechnology, and biochemistry,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
June 2000, Experimental hematology,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
April 2017, Nucleic acids research,
Chunye Lu, and Zeping Luo, and Stefanie Jäger, and Nevan J Krogan, and B Matija Peterlin
August 1991, Journal of virology,
Copied contents to your clipboard!