Pre- and postembedding immunocytochemical procedures were used, together with antisera raised against oxytocin or its neurophysin, to characterize oxytocinergic pathways in the rat spinal cord, at the electron microscopic level. Pre-embedding immunoperoxidase staining performed on vibratome sections revealed oxytocin- and neurophysin-positive axonal profiles and terminals scattered predominantly in laminae I and II of the dorsal horn and in the central gray (lamina X). They were also visible, but to a lesser extent, in the intermediolateral columns, at thoracic and lumbar levels. Postembedding immunogold staining performed directly on ultrathin sections of the same areas, fixed in osmium and embedded in resin, permitted to show clearly that the oxytocinergic axons made symmetrical and asymmetrical synaptic contacts onto dendritic profiles. It also allowed subcellular localization of the neuropeptide immunoreactivities which were restricted to relatively large, electron-dense vesicles in the immunopositive terminals. Oxytocinergic terminals were never seen to participate in glomerular configurations in the superficial layers of the dorsal horn nor were immunoreactive cell bodies visible in any spinal area. Our results provide direct morphological evidence that oxytocinergic pathways make synapses in several regions of the spinal cord, thus supporting the contention that oxytocin may exert neurotransmitter/neuromodulator actions in this area of the CNS.