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Natural killer (NK) cells (CD3-) or large granular lymphocytes (LGL) spontaneously kill K562 targets but are unable to kill Daudi cells in the absence of IL-2 stimulation. IL-4 is reported to prevent or inhibit the IL-2-driven lymphokine-activated killer (LAK) generation in NK cells. Therefore, we wished to determine whether the antagonistic effect of IL-4 on IL-2-induced LAK activity might regulate the expression of genes encoding proteins involved in lysis, such as perforin, the pore-forming protein, or which are associated with lysis, such as granzymes A and B. By using in situ hybridization, we showed that, in addition to inducing LAK activity, IL-2 stimulation increased the amount of perforin and granzyme B mRNA at the single-cell level in 40 to 100% of the total CD3- LGL cell population. In addition, our results indicated that the stimulatory effect of IL-2 can be downregulated by IL-4 for both LAK activity and granzyme B and perforin gene expression. Here again, a decrease in the amount of specific mRNA per cell was noted. These findings suggest that modulation of the lytic machinery via lymphokines might be associated with regulation of the lytic potential of NK cells.
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P Haddad, and
A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
January 1990,
Nouvelle revue francaise d'hematologie,
M V Clément, and
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S Legros-Maida, and
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M Sasportes
November 2005,
Environmental toxicology and pharmacology,
M V Clément, and
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A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
September 1993,
Journal of immunology (Baltimore, Md. : 1950),
M V Clément, and
P Haddad, and
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S Legros-Maida, and
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M Sasportes
August 2004,
Toxicology,
M V Clément, and
P Haddad, and
A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
February 2011,
Blood,
M V Clément, and
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S Legros-Maida, and
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December 1998,
Liver,
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June 1999,
Cellular immunology,
M V Clément, and
P Haddad, and
A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
October 2002,
Journal of leukocyte biology,
M V Clément, and
P Haddad, and
A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
January 2020,
OncoTargets and therapy,
M V Clément, and
P Haddad, and
A Soulié, and
S Legros-Maida, and
J Guillet, and
E Cesar, and
M Sasportes
March 2000,
Brain, behavior, and immunity,
